bs-1305R
[Primary Antibody]
CCR7/CD197 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CCR7/CD197
Immunogen Range: 25-59/379
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 12775
Swiss Prot: P47774
Source: KLH conjugated synthetic peptide derived from mouse CCR7
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
CCR7 is a member of the G protein coupled receptor family (subfamily : chemokine). This receptor was identified as a gene induced by the Epstein Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. CCR7 has been reported to be expressed in blood, bone marrow, lymph node, and intestine. It is particularly expressed in lymphoid tissues and in activated B and T lymphocytes and has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. ESTs have been isolated from blood, embryo, lymph node, and thymus libraries.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 42
Cross Reactive Species:
Human
Mouse
Rat
Sheep
Predicted Cross Reactive Species: Dog
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- en Menachem-Zidon O et al. Age-associated differences in macrophage response in a vaginal wound healing rat model. Int Urogynecol J. 2020 Feb 27.Read more>>
- Blomgran, Parmis, et al. "A possible link between loading, inflammation and healing: Immune cell populations during tendon healing in the rat." Scientific Reports 6 (2016): 29824.Read more>>
- "Li, Xuelu, et al. ""High Expression of CCR7 Predicts Lymph Node Metastasis and Good Prognosis in Triple Negative Breast Cancer."" Cellular Physiology and Biochemistry 43.2 (2017): 531-539. "Read more>>
- Dijk et al. Systematic Evaluation of the Cellular Innate Immune Response During the Process of Human Atherosclerosis. (2016) J.Am.Heart.Assoc. 5 Read more>>
- Expression of Pref-1 and Related Chemokines during theDevelopment of Rat Mesenteric Lymph Nodes.(2018)Biomed Environ Sci.ul;31(7):507-514. Read more>>
- Liu Y et al. Isolation and characterization of ovine monocyte-derived macrophages from peripheral blood.(2018)Vet Immunol Immunopathol. Nov;205:83-92. Read more>>
- Li Y et al. Role of CCR7 on dendritic cell‑mediated immune tolerance in the airways of allergy‑induced asthmatic rats. Mol Med Rep. 2019 Sep 20. Read more>>
- Ben Menachem-Zidon O et al. Age-associated differences in macrophage response in a vaginal wound healing rat model. Int Urogynecol J. 2020 Feb 27.Read more>>
- Liu Xingdan. et al. Hydroxyapatite composited PEEK with 3D porous surface enhances osteoblast differentiation through mediating NO by macrophage. Regen Biomater. 2021 DecRead more>>
- Hao, Yanan. et al. Gut microbiota-testis axis: FMT improves systemic and testicular micro-environment to increase semen quality in type 1 diabetes. MOL MED. 2022 Dec;28(1):1-17Read more>>
- Zheng Xinhui. et al. Effect of micro/nano-sheet array structures on the osteo-immunomodulation of macrophages. REGEN BIOMATER. 2022 OcRead more>>
- Hanhan Fang. et al. Gut-Spleen Axis: Microbiota via Vascular and Immune Pathways Improve Busulfan-Induced Spleen Disruption | mSphere. MSPHERE. 2022 DecRead more>>
- Chengetai R Mahomva. et al. Chemokine CCL19 and Its Receptors CCR7 and CCRL1 in Chronic Rhinosinusitis. J INFLAMM RES. 2024 May;17:2991-3002Read more>>
- Ao Wang. et al. Inhibition of NLRP3 inflammasome ameliorates LPS-induced neuroinflammatory injury in mice via PINK1/Parkin pathway. NEUROPHARMACOLOGY. 2024 Oct;257:110063Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti CCR7/CD197 Polyclonal Antibody, Unconjugated (bs-1305R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti CCR7/CD197 Polyclonal Antibody, Unconjugated (bs-1305R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy5 conjugated(bs-0295G-Cy5)used at 1:200 dilution
Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Rabbit Anti-CCR7/CD197 Polyclonal Antibody, Unconjugated (bs-1305R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Raji probed with CCR7/CD197 Polyclonal Antibody, Unconjugated (bs-1305R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CCR7/CD197 Polyclonal Antibody(bs-1305R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat lymph node; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CCR7/CD197 Polyclonal Antibody, Unconjugated (bs-1305R) at 1:200 overnight at 4°C, DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CCR7) polyclonal Antibody, Unconjugated (bs-1305R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.