bs-1301R [Primary Antibody]
FGFR3/CD333 Polyclonal Antibody
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Host: Rabbit

Target Protein: FGFR3/CD333

Immunogen Range: 501-600/801


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2261

Source: KLH conjugated synthetic peptide derived from human FGFR3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 86


Cross Reactive Species: Human

Predicted Cross Reactive Species: Dog
Cow
Sheep
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lu, Jiang, Kehuan Lu, and Dongsheng Li. "Changes in expression and secretion patterns of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway molecules during murine neural stem/progenitor cell differentiation in vitro***??.Neural Regen Res. 2012;7(22):1688-1694.Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Anti-FGFR3/CD333 Polyclonal Antibody, Unconjugated (bs-1301R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Human MCF-7 cell lysates; Lane 2: Human HeLa cell lysates probed with FGFR3 Polyclonal Antibody, Unconjugated (bs-1301R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.