DATASHEET
Host:
Rabbit
Target Protein:
CYP2A/Cytochrome P450 2A6
Immunogen Range:
388-494/494
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
1548
Source:
KLH conjugated synthetic peptide derived from human CYP2A/Cytochrome P450 2A6
Purification:
Purified by Protein A.
Storage Buffer:
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds (1). Several P450 enzymes have been classified by sequence similarities as members of the CYP1A and CYP2A subfamilies (2). NADPH cytochrome P450 reductase is a microsomal enzyme responsible for the transfer of electrons from NADPH to cytochrome P450 enzymes during the P450 catalytic cycle (3,4). NADPH cytochrome P450 reductase is localized to the endoplasmic reticulum where it is also able to transfer electrons to heme oxygenase and cytochrome b5 (5,6). NADPH cytochrome P450 reductase is structurally related to two separate flavoprotein families, ferredoxin nucleotide reductase (FNR) and flavodoxin (7). Electron transfer of NADPH cytochrome P450 reductase requires the binding of two flavin cofactors, FAD and FMN, to the FNR and flavodoxin domains, respectively (8).
Conjugation:
Biotin
Excitation/ Emission:
N/A
Size:
100ul
Concentration:
1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
56
Predicted Cross Reactive Species:
Human
Mouse
Rat
Dog
Cow
Sheep
For research use only. Not intended for diagnostic or therapeutic use.