bs-12911R [Primary Antibody]
c-Fos (Ser374) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: c-Fos Ser374

Modification Site: Ser374

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2353

Source: KLH conjugated synthetic phosphopeptide derived from human c-Fos around the phosphorylation site of Ser374

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 41


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu, Jing-Jing, et al. "A novel AP-1/miR-101 regulatory feedback loop and its implication in the migration and invasion of hepatoma cells." Nucleic Acids Research (2014): gku872.Read more>>
VALIDATION IMAGES

Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates probed with Phospho-c-Fos (Ser374) Polyclonal Antibody, Unconjugated (bs-12911R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.