bs-12867R [Primary Antibody]
Bile Acid Receptor NR1H4 Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Bile Acid Receptor NR1H4

Immunogen Range: 175-280/486


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9971

Swiss Prot: Q96RI1

Source: KLH conjugated synthetic peptide derived from human Bile Acid Receptor NR1H4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The steroid receptor superfamily acts through direct association with DNA sequences known as hormone response elements (HREs) and binds DNA as either homo- or heterodimers. The promiscuous mediator of heterodimerization, RXR, is the receptor for 9-cis retinoic acid, and dimerizes with VDR, TR, PPAR, and several novel receptors including LXR (also referred to as RLD-1) and FXR. FXR and LXR fall into a category of proteins termed orphan receptors? because of their lack of a defined function, and in the case of LXR, the lack of a defined ligand. FXR has been shown to bind a class of lipid molecules called farnesoids. LXR/RXR heterodimers have highest affinity for DR-4 DNA elements while FXR/RXR heterodimers bind IR-1 elements. Both LXR/RXR and FXR/RXR heterodimers retain their responsiveness to 9-cis retinoic acid.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 56


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Sheep
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Guo, Hong-li, et al. "Pyrazinamide induced rat cholestatic liver injury through inhibition of FXR regulatory effect on bile acid synthesis and transport."Toxicological Sciences (2016): kfw098.Read more>>
  • Li, Xiaojiaoyang, et al. "UDCA and CDCA alleviate 17α-ethinylestradiol-induced cholestasis through PKA-AMPK pathways in rats." Toxicology and Applied Pharmacology 311 (2016): 12-25.Read more>>
  • Yu, Linxi, et al. "Protective effects of SRT1720 via the HNF1α/FXR signalling pathway and anti-inflammatory mechanisms in mice with estrogen-induced cholestatic liver injury." Toxicology Letters (2016).Read more>>
  • Zhang, Qiankun, et al. "Effects of the fibrous topography-mediated macrophage phenotype transition on the recruitment of mesenchymal stem cells: An in vivo study." Biomaterials (2017)Read more>>
  • Yang, Tingting, et al. "Early indications of ANIT-induced cholestatic liver injury: Alteration of hepatocyte polarization and bile acid homeostasis." Food and Chemical Toxicology (2017).Read more>>
  • Yu et al. SRT1720 Alleviates ANIT-Induced Cholestasis in a Mouse Model. (2017) Front.Pharmaco. 8:256Read more>>
  • Zhou et al. Retinoic acid induces macrophage cholesterol efflux and inhibits atherosclerotic plaque formation in apoE-deficient mice. (2015) Br.J.Nutr. 114:509-18Read more>>
  • Zhao G et al. Adaptive homeostasis of the vitamin D–vitamin D nuclear receptor axis in 8-methoxypsoralen-induced hepatotoxicity. (2018) Toxicology and Applied Pharmacology. Jan 1;362:150-158. Read more>>
  • Liu Y et al. Fish oil alleviates circadian bile composition dysregulation in male mice with NAFLD. J Nutr Biochem. 2019 Apr 4;69:53-62.Read more>>
  • Yuan Z et al. A new perspective of triptolide-associated hepatotoxicity: Liver hypersensitivity upon LPS stimulation. Toxicology. 2019 Feb 15;414:45-56. Read more>>
  • Li T et al. Picroside II protects against cholestatic liver injury possibly through activation of farnesoid X receptor. Phytomedicine,2019. 153153. Read more>>
  • Yong-li Hua. et al. Baitouweng Tang ameliorates DSS-induced ulcerative colitis through the regulation of the gut microbiota and bile acids via pathways involving FXR and TGR5. Biomed Pharmacother. 2021 May;137:11132Read more>>
  • Mengzhi Zou. et al. The role of invariant natural killer T cells and associated immunoregulatory factors in triptolide-induced cholestatic liver injury. Food Chem Toxicol. 2020 Dec;146:111777Read more>>
  • Chen Lisheng. et al. Paeoniflorin Protects against ANIT-Induced Cholestatic Liver Injury in Rats via the Activation of SIRT1-FXR Signaling Pathway. Evid-Based Compl Alt. 2021;2021:8479868Read more>>
  • Fan Yadong. et al. Abnormal bile acid metabolism is an important feature of gut microbiota and fecal metabolites in patients with slow transit constipation. FRONT CELL INFECT MI. 2022 Jul;0:106Read more>>
  • Zhang, Yaxin. et al. Stigmasterol attenuates hepatic steatosis in rats by strengthening the intestinal barrier and improving bile acid metabolism. npj Science of Food. 2022 Aug;6(1):1-14Read more>>
  • Jie Wang. et al. Obeticholic acid aggravates liver injury by up-regulating the liver expression of osteopontin in obstructive cholestasis. LIFE SCI. 2022 Oct;307:120882Read more>>
  • Kong, Weichao. et al. iNKT17 cells play a pathogenic role in ethinylestradiol-induced cholestatic hepatotoxicity. ARCH TOXICOL. 2022 Nov;:1-2Read more>>
  • Peishi Liang. et al. Obeticholic acid improved triptolide/lipopolysaccharide-induced hepatotoxicity by inhibiting Caspase-11-GSDMD pyroptosis pathway. J APPL TOXICOL. 2022 NovRead more>>
  • Xiaoyue Li. et al. Dietary bile acids promote sterol metabolism, bile acids enterohepatic circulation, and apoptosis in juvenile Pacific white shrimp (Litopenaeus vannamei). ANIM FEED SCI TECH. 2023 Sep;303:11571Read more>>
  • Menglin Shi. et al. Effects of Dietary Chenodeoxycholic Acid Supplementation in a Low Fishmeal Diet Containing Clostridium autoethanogenum Protein on Growth, Lipid and Cholesterol Metabolism, and Hepatopancreas Health of Litopenaeus vannamei. ANIMALS. 2023 Jan;13(13):2109Read more>>
  • Mei-Qi Wang. et al. Wedelolactone alleviates cholestatic liver injury by regulating FXR-bile acid-NF-B/NRF2 axis to reduce bile acid accumulation and its subsequent inflammation and oxidative stress. PHYTOMEDICINE. 2023 Sep;:155124Read more>>
  • Liutong Chen. et al. Effects of replacing fishmeal with different proportions of mixed protein source in the diet of largemouth bass (Micropterus salmoides). COMP BIOCHEM PHYS D. 2024 Mar;49:101181Read more>>
  • Dongmin Yang. et al. Farnesoid X Receptor Protects Murine Lung Against IL-6-promoted Ferroptosis Induced by Poly(I:C). AM J RESP CELL MOL. 2024 Feb 01Read more>>
  • Xiao Ying. et al. Pulsatilla decoction alleviates DSS-induced UC by activating FXR-ASBT pathways to ameliorate disordered bile acids homeostasis. FRONT PHARMACOL. 2024 Jun;15Read more>>
  • Chen Liang. et al. Fluoride induces hepatointestinal damage and vitamin B2 mitigation by regulating IL-17A and Bifidobacterium in ileum. J ADV RES. 2024 AugRead more>>
  • Chen Liutong. et al. A mixed animal and plant protein source replacing fishmeal affects bile acid metabolism and apoptosis in largemouth bass (Micropterus salmoides). J ANIM SCI. 2024 AugRead more>>
VALIDATION IMAGES

Lane 1: HepG2 lysates probed with Bile Acid Receptor NR1H4 Antibody (bs-12867R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 90 min at 37˚C.


Mouse liver lysates probed with Bile Acid Receptor NR1H4 Polyclonal Antibody, Unconjugated (bs-12867R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Pancreas tissue lysates; Lane 3: Rat Liver tissue lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human MCF-7 cell lysates probed with NR1H4 Polyclonal Antibody, Unconjugated (bs-12867R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.