bs-1283R [Primary Antibody]
ASPP2/p53BP2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ASPP2/p53BP2

Immunogen Range: 1001-1128/1128


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7159

Swiss Prot: Q13625

Source: KLH conjugated synthetic peptide derived from human ASPP2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)

Predicted Molecular Weight: 124


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xie, Xiao-Feng, et al. "Prognostic values of apoptosis-stimulating P53-binding protein 1 and 2 and their relationships with clinical characteristics of esophageal squamous cell carcinoma patients: a retrospective study." Chinese Journal of Cancer 36.1 (2017): 15.Read more>>
  • Wang CH et al. LncRNA ANRIL promotes cell proliferation, migration and invasion during acute myeloid leukemia pathogenesis via negatively regulating miR-34a. Int J Biochem Cell Biol. 2020 Feb;119:105666.Read more>>
VALIDATION IMAGES

Lane 1: Mouse spleen lysate Lane 2: Mouse thymus lysate Lane 3: Mouse prostate lysate Lane 4: Mouse intestine lysate probed with Rabbit Anti-ASPP2/p53BP2 Polyclonal Antibody, Unconjugated (bs-1283R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.


Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti-ASPP2/p53BP2 Polyclonal Antibody, Unconjugated (bs-1283R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ASPP2 ) Polyclonal Antibody, Unconjugated (bs-1283R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.