Host: Rabbit
Target Protein: E-Selectin/CD62E
Immunogen Range: 501-612/612
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 20339
Swiss Prot: Q00690
Source: KLH conjugated synthetic peptide derived from mouse E-Selectin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 65
Cross Reactive Species:
Human
Mouse
Rat
Pig
Predicted Cross Reactive Species:
Dog
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
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Formalin-fixed and paraffin embedded human gastric carcinoma tissue labeled with Anti-E-Selectin Polyclonal Antibody, Unconjugated(bs-1273R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric carcinoma tissue labeled with Anti-E-Selectin Polyclonal Antibody, Unconjugated(bs-1273R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
100 ng rhE-Selectin-His protein (bs-43646P) per lane probed with E-Selectin polyclonal antibody respectively, unconjugated (bs-1273R) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with E-Selectin Polyclonal Antibody, Unconjugated (bs-1273R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.