Host: Rabbit
Target Protein: CCL20/MIP3 alpha
Immunogen Range: 31-97/97
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 20297
Swiss Prot: O89093
Source: KLH conjugated synthetic peptide derived from mouse CCL20
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 8.6
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species: Rat
For research use only. Not intended for diagnostic or therapeutic use.
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- Qin, Shan-Yu, et al. "Role and recruitment of Th9 cells in liver cirrhosis patients." Asian Pacific Journal of Tropical Biomedicine (2016).Read more>>
- Liu et al. Clara cell 10-kDa protein inhibits T(H)17 responses through modulating dendritic cells in the setting of allergic rhinitis. (2013) J.Allergy.Clin.Immunol. 131:387-94.e1-12Read more>>
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- Chuan Chen. et al. A novel small molecule effectively ameliorates estrogen deficiency-induced osteoporosis by targeting the gut-bone signaling axis. EUR J PHARMACOL. 2023 Sep;954:175868Read more>>
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti-CCL20/MIP3 alpha Polyclonal Antibody, Unconjugated (bs-1268R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Paraformaldehyde-fixed, paraffin embedded human liver carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CCL20 Polyclonal Antibody, Unconjugated (bs-1268R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.