bs-12550R [Primary Antibody]
ATPase Inhibitory Factor 1 Antibody
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DATASHEET

Host: Rabbit

Target Protein: ATP1/ATPase Inhibitory Factor 1

Immunogen Range: 26-106/106


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 93974

Source: KLH conjugated synthetic peptide derived from human ATPIF1/ATPase Inhibitory Factor 1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. F1 consists of three Alpha chains and three Beta chains, which are weakly homologous, as well as one Gamma chain, one Delta chain and one Gamma chain. F0 consists of three subunits: a, b and c. A mitochondrial F1-ATPase inhibitor protein, ATPIF1 (ATPase inhibitory factor 1), also known as IP, IF1, ATPI or ATPIP (ATPase inhibitor protein), binds to the C-terminal region of a Beta subunit of the F1-ATPase at low pH values and, via interference of the Beta and Gamma subunit interaction, ATPIF1 regulates the activity of the F1F0-ATPase. This reversible ATPIF1 binding to F1F0-ATPase also occurs on the surface of endothelial cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:500-2000)
ELISA(1:5000-10000)

Predicted Molecular Weight: 10


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Human HeLa cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Human U937 cell lysates; Lane 4: Human 293T cell lysates probed with ATPIF1 Polyclonal Antibody, Unconjugated (bs-12550R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.