bs-12531R [Primary Antibody]
TSPAN32 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PHEMX/ART1

Immunogen Range: 101-200/320


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 10077

Source: KLH conjugated synthetic peptide derived from human TSPAN32

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Phemx is a member of the tetraspanin (TM4SF) family of proteins that may be involved in transmembrane signal transduction, regulation of cell proliferation, differentiation and motility. Phemx is a multi-pass membrane protein containing intracellular N- and C-terminal domains, four transmembrane domains and two extracellular loops. It is ubiquitously expressed from early embryogenesis through adulthood. Phemx exhibits predominant expression in hematopoietic tissues suggesting a role in hematopoietic-cell function. In association with the Integrin IIb/Integrin _3 complex, Phemx functions to stabilize arterial thrombi in platelets and regulate outside-in? signaling. This interaction may be important in the process of wound healing. The gene encoding Phemx is located in an important tumor-suppressor gene region that has been associated with Beckwith-Wiedemann syndrome as well as a variety of cancers.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
IP(1-2ug)
ICC(1:100-500)

Predicted Molecular Weight: 35


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Tang et al. ART1 promotes starvation-induced autophagy: a possible protective role in the development of colon carcinoma. (2015) Am.J.Cancer.Res. 5:498-513Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TSPAN32 Polyclonal Antibody, Unconjugated (bs-12531R) at 1:200 overnight at 4°C, DAB staining.