bs-1251R [Primary Antibody]
CD166 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD166

Immunogen Range: 451-583/583


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 214

Swiss Prot: Q13740

Source: KLH conjugated synthetic peptide derived from human CD166

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 62


Cross Reactive Species: Human
Rabbit

Predicted Cross Reactive Species: Mouse
Rat
Dog
Cow
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lee, Tao-Chen, et al. "Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells." PLOS ONE 9.11 (2014): e111390.Read more>>
  • Kovac, Michal, et al. "Different RNA and protein expression of surface markers in rabbit amniotic fluid‐derived mesenchymal stem cells." Biotechnology Progress (2017).Read more>>
  • Vašíček J et al. Combined approach for characterization and quality assessment of rabbit bone marrow-derived mesenchymal stem cells intended for gene banking. N Biotechnol. 2019 Aug 7;54:1-12.Read more>>
  • Wenping Luo. et al. BMP9\initiated osteogenic/odontogenic differentiation of mouse tooth germ mesenchymal cells (TGMCS) requires Wnt/\catenin signalling activity. 2021 Feb 18Read more>>
  • Jaromr Va??ek. et al. Molecular Profiling and Gene Banking of Rabbit EPCs Derived from Two Biological Sources. Genes-Basel. 2021 Mar;12(3):366Read more>>
  • Yue Wu. et al. LncRNA WDFY3-AS2 promotes cisplatin resistance and the cancer stem cell in ovarian cancer by regulating hsa-miR-139-5p/SDC4 axis. Cancer Cell Int. 2021 Dec;21(1):1-14Read more>>
  • Zhen Zhou. et al. Myogenic Determination and Differentiation of Chicken Bone Marrow-Derived Mesenchymal Stem Cells under Different Inductive Agents. ANIMALS. 2022 Jan;12(12):1531Read more>>
  • Qianyu Cheng. et al. Establishing and characterizing human stem cells from the apical papilla immortalized by hTERT gene transfer. FRONT CELL DEV BIOL. 2023; 11: 1158936Read more>>
  • Yue Wu. et al. The biological characteristics of chicken embryo mesenchymal stem cells isolated from chorioallantoic membrane. GENESIS. 2024 Apr;62(2):e23592Read more>>
VALIDATION IMAGES

Lane 1: human colon carcinoma lysates Lane 2: rat brain lysates probed with Anti CD166 Polyclonal Antibody, Unconjugated (bs-1251R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 64kD. Observed band size: 64kD.


Molt4 cells probed with CD166 Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated (bs-1251R-A488) at 0.2ug for 30 minutes compared to control cells (blue) and isotype control (orange).