bs-1243R [Primary Antibody]
TPM2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TPM2

Immunogen Range: 11-130/284


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7169

Swiss Prot: P07951

Source: KLH conjugated synthetic peptide derived from human TPM2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform may have a role in agonist-mediated receptor internalization.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 33


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin-embedded mouse stomach labeled with TPM2 Polyclonal Antibody, Unconjugated (bs-1243R) at 1:200 followed by incubation with secondary antibody and DAB staining


Formalin-fixed and paraffin-embedded human breast carcinoma labeled with TPM2 Polyclonal Antibody, Unconjugated (bs-1243R) at 1:200 followed by incubation with secondary antibody and DAB staining


Lane 1: Muscle lysates probed with TPM2 Polyclonal Antibody, Unconjugated (bs-1243R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Small intestine lysates probed with TPM2 Polyclonal Antibody, Unconjugated (bs-1243R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.