bs-12418R [Primary Antibody]
BAMBI Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: BAMBI

Immunogen Range: 101-200/260


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 25805

Swiss Prot: Q13145

Source: KLH conjugated synthetic peptide derived from human BAMBI

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

BAMBI is a membrane spanning glycoprotein that acts as a negative regulator of TGF-beta signaling during development. The BAMBI family of proteins are related to type I TGF-beta receptor family, however, BAMBI is a pseudoreceptor that lacks an intracellular serine/threonine kinase domain. BAMBI transcription regulation is under the influence of beta-catenin, BMP, smad3 and smad4. BAMBI expression can increase in colorectal and hepatocellular carcinomas relative to non-cancerous tissues. BAMBI is expressed at high levels during odontogenesis. BAMBI is coexpressed with Bmp-4 during early Xenopus embryogenesis and can be detected in poorly metastatic human melanoma cell lines.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 27


Cross Reactive Species: Human
Mouse
Rat
Pig

Predicted Cross Reactive Species: Dog
Cow
Sheep
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Bai, Long, et al. "Identification and Expression Analyses of BAMBI Mediated by FSH in Swine Luteinizing Granulosa Cells." Theriogenology (2014).Read more>>
  • He, Yao, et al. "LPS/TLR4 Signaling Enhances TGF-β Response Through Downregulating BAMBI During Prostatic Hyperplasia." Scientific reports 6 (2016): 27051.Read more>>
  • Du, Jinghua, et al. "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." Molecular medicine reports 13.3 (2016): 2159-2166.Read more>>
  • Lu H.J., et al. “Mechanism of MicroRNA-708 Targeting BAMBI in Cell Proliferation, Migration, and Apoptosis in Mice With Melanoma via the Wnt and TGF-β Signaling Pathways.” Technology in Cancer Research & Treatment (2018); 17: 1-15.Read more>>
  • Hongwei Chen. et al. Dissecting Heterogeneity Reveals a Unique BAMBIhighMFGE8high Subpopulation of Human UC-MSCs. Advanced Science. 2022 Nov;:220251Read more>>
  • Hongwei Chen. et al. Dissecting Heterogeneity Reveals a Unique BAMBIhighMFGE8high Subpopulation of Human UC-MSCs. Advanced Science. 2022 Nov;:220251Read more>>
  • Yaqi Zhang. et al. Effects of BAMBI on luteinized follicular granulosa cell proliferation and steroid hormone production in sheep. MOL REPROD DEV. 2023 FebRead more>>
VALIDATION IMAGES

L1 rat brain lysates L2 rat kidney lysates probed with Anti BAMBI Polyclonal Antibody, Unconjugated (bs-12418R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 29kD. Observed band size:29kD.


Paraformaldehyde-fixed, paraffin embedded Human Kidney Tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with BAMBI Polyclonal Antibody, Unconjugated (bs-12418R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.


Lane 1: Human K562 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human A549 cell lysates probed with BAMBI Polyclonal Antibody, Unconjugated (bs-12418R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.