DATASHEET
Host:
Rabbit
Target Protein:
AMHR2
Immunogen Range:
21-120/573
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
269
Source:
KLH conjugated synthetic peptide derived from human MISRII/AMHR2
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
MISR II is a 573 amino acid protein encoded by the human gene AMHR2. MISR II belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family, TGFB receptor subfamily and contains one protein kinase domain. Upon ligand binding, MISR II forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. These type II receptors rylate and activate type I receptors which autophosphorylate, then bind and activate Smad transcriptional regulators. MISR II also acts as a receptor for anti-Muellerian hormone. Defects in AMHR2 are the cause of persistent Muellerian duct syndrome type 2 (PMDS-2). PMDS-2 is a form of male pseudo-hermaphroditism characterized by a failure of Muellerian duct regression in otherwise normal males.
PRODUCT SPECIFIC PUBLICATIONS
- Huang CC et al. Hyperactive CREB signaling pathway involved in the pathogenesis of polycystic ovarian syndrome revealed by patient-specific induced pluripotent stem cell modeling. Fertil Steril. 2019 Jul 2. pii: S0015-0282(19)30428-5. Read more>>
VALIDATION IMAGES
Human jurkat cells probed with MISRII/AMHR2 Polyclonal Antibody, Unconjugated (bs-12414R) (green) at 1:20 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
293T cell lysates probed with AMHR2 Polyclonal Antibody, Unconjugated (bs-12414R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Adrenal gland tissue lysates; Lane 2: Rat Adrenal gland tissue lysates probed with AMHR2 Polyclonal Antibody, Unconjugated (bs-12414R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.