bs-12364R [Primary Antibody]
SCXA/Scleraxis Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SCXA/Scleraxis

Immunogen Range: 165-200/201


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 642658

Swiss Prot: Q7RTU7

Source: KLH conjugated synthetic peptide derived from human SCXA/Scleraxis

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 22


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Dog
Cow
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ruidong Ge. et al. Quantitative proteomics reveals potential anti-inflammatory protein targets of radial extracorporeal shock wave therapy in TNF--induced model of acute inflammation in primary human tenocytes. HELIYON. 2022 Dec;8:e12008Read more>>
  • Kang Lu. et al. Micropattern Silk Fibroin Film Facilitates Tendon Repair In Vivo and Promotes Tenogenic Differentiation of Tendon Stem/Progenitor Cells through the 21/FAK/PI3K/AKT Signaling Pathway In Vitro. STEM CELLS INT. 2023 Jan 13;2023:2915826Read more>>
  • Lu, Kang. et al. N-Acetyl-L-cysteine facilitates tendon repair and promotes the tenogenic differentiation of tendon stem/progenitor cells by enhancing the integrin 5/1/PI3K/AKT signaling. BMC MOL CELL BIOL. 2023 Dec;24(1):1-13Read more>>
  • Li Zhi. et al. Dynamic Tensile Stress Promotes Regeneration of Achilles Tendon in a Panda Rope Bridge Technique Mice Model. ANN BIOMED ENG. 2023 Jul;:1-14Read more>>
  • Lu Tan. et al. Mechanically Robust Hemostatic Hydrogel Membranes with Programmable Strain-Adaptive Microdomain Entanglement for Wound Treatment in Dynamic Tissues. ACS NANO. 2024;XXXX(XXX):XXX-XXXRead more>>
  • Jiu-Jiu Zhang. et al. Harnessing Mechanical Stress with Viscoelastic Biomaterials for Periodontal Ligament Regeneration. ADV SCI. 2024 Mar;:2309562Read more>>
  • Xuan Yao. et al. Dual Dynamic Crosslinked Hydrogel Patch Embodied with Anti-Bacterial and Macrophage Regulatory Properties for Synergistic Prevention of Peritendinous Adhesion. ADV FUNCT MATER. 2024 May;:240066Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-SCXA/Scleraxis Polyclonal Antibody, Unconjugated (bs-2986R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat thyroid labeled with Anti-SCXA/Scleraxis Polyclonal Antibody, Unconjugated (bs-12364R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded rat muscle tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Scleraxis Polyclonal Antibody, Unconjugated (bs-12364R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining