bs-12358R [Primary Antibody]
YKL39/CHI3L2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
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DATASHEET

Host: Rabbit

Target Protein: YKL39/CHI3L2

Immunogen Range: 121-220/390


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7690

Source: KLH conjugated synthetic peptide derived from human YKL39/CHI3L2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

YKL-39 is a 390 amino acid cartilage protein that belongs to the chitinase family of chitin-fragmenting hydrolases. Highly expressed in chondrocytes (cartilage cells) and synoviocytes (fibroblastic cells that line joint cavities), YKL-39 binds glycan structures with high affinity. Although related to bacterial chitinases, YKL-39 lacks the characteristic glutamate active site and, thus, does not have enzymatic chitinase activity. Patients affected with rheumatoid arthritis (RA) have autoimmunity against YKL-39, suggesting that YKL-39 is involved in osteoarthritic and/or rheumatoid joint disease. Additionally, YKL-39 is upregulated in early degenerative cartilage diseases (such as RA) and may be a marker of chondrocyte activation in these autoimmune conditions.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 41


Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xue Ling. et al. YKL-39 is an independent prognostic factor in gastric adenocarcinoma and is associated with tumor-associated macrophage infiltration and angiogenesis. WORLD J SURG ONCOL. 2022 Dec;20(1):1-9Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with YKL39/CHI3L2 Polyclonal Antibody, Unconjugated (bs-1238R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.