bs-1231R-Cy5.5 [Conjugated Primary Antibody]
ABCG1 Polyclonal Antibody, Cy5.5 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ABCG1

Immunogen Range: 561-678/678


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9619

Swiss Prot: P45844

Source: KLH conjugated synthetic peptide derived from human ABCG1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.

Conjugation: Cy5.5

Excitation/ Emission: 675nm/694nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 76


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jia, Lianqun, et al. "Effects of Tanshinone IIA on the modulation of miR‑33a and the SREBP‑2/Pcsk9 signaling pathway in hyperlipidemic rats." Molecular Medicine Reports (2016).Read more>>
  • Zhang Y et al. Exposure to carbon black nanoparticles during pregnancy persistently damages the cerebrovascular function in female mice. Toxicology. 2019 Apr 22;422:44-52.Read more>>
  • Yang B et al. Polychlorinated Biphenyl Quinone Promotes Macrophage-Derived Foam Cell Formation. Chem Res Toxicol. 2019 Nov 13.Read more>>
VALIDATION IMAGES

Molt4 cells(black) were fixed with 4% PFA for 10min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ABCG1 Polyclonal Antibody, Cy5.5 Conjugated (bs-1231R-Cy5.5) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).