Host: Rabbit
Target Protein: Adenosine A3 Receptor
Immunogen Range: 85-180/347
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 140
Swiss Prot: P0DMS8
Source: KLH conjugated synthetic peptide derived from human ADORA3
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 35
Cross Reactive Species:
Human
Rat
Predicted Cross Reactive Species:
Mouse
Dog
Cow
Sheep
Pig
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
Formalin-fixed and paraffin embedded human gastric carcinoma tissue labeled with Anti-ADORA3/Adenosine A3 Receptor Polyclonal Antibody, Unconjugated(bs-1225R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: human colon carcinoma lysates Lane 2: human gastric carcinoma lysates probed with Anti ADORA3 Polyclonal Antibody, Unconjugated (bs-1225R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 35kD. Observed band size: 35kD
Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Bone tissue lysates; Lane 3: Rat Heart tissue lysates probed with Adenosine A3 Receptor Polyclonal Antibody, Unconjugated (bs-1225R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HL-60 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Adenosine A3 Receptor Antibody(bs-1225R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).