bs-1223R [Primary Antibody]
Aggrecan core protein Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Aggrecan core protein 1

Immunogen Range: 101-220/2415


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 176

Swiss Prot: P16112

Source: KLH conjugated synthetic peptide derived from human Aggrecan core protein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 208/248


Cross Reactive Species: Human
Mouse
Rat
Rabbit

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lei, Ming, et al. "Mesenchymal stem cell characteristics of dental pulp and periodontal ligament stem cells after< i> in vivo</i> transplantation." Biomaterials (2014).Read more>>
  • Shi, Yang, et al. "Hypoxia combined with spheroid culture improves cartilage specific function in chondrocytes." Integrative Biology (2015).Read more>>
  • Liu, Yong, et al. "Lentivirus-mediated TGF-β3, CTGF and TIMP1 gene transduction as a gene therapy for intervertebral disc degeneration in an in vivo rabbit model." Experimental and Therapeutic Medicine.Read more>>
  • Zhao, Yangfei, et al. "Effect of Choline on the Composition and Degradation Enzyme of Extracellular Matrix of Mice Chondrocytes Exposed to Fluoride."Biological Trace Element Research (2016): 1-7.Read more>>
  • Hu, Ming‐Hsiao, et al. "Optimization of Puncture Injury to Rat Caudal Disc for Mimicking Early Degeneration of Intervertebral Disc." Journal of Orthopaedic Research (2017).Read more>>
  • Xu, Xilin, et al. "Danshen attenuates osteoarthritis-related cartilage degeneration through inhibition of NF-κB signaling pathway in vivo and in vitro." Biochemistry and Cell Biology (2017).Read more>>
  • Ren et al. Treatment of rabbit intervertebral disc degeneration with co-transfection by adeno-associated virus-mediated SOX9 and osteogenic protein-1 double genes in vivo. (2013) Int.J.Mol.Me. 32:1063-8Read more>>
  • Yang et al. miR-1307-3p suppresses the chondrogenic differentiation of human adipose-derived stem cells by targeting BMPR2. (2018) Int.J.Mol.Med. 42:3115-3124Read more>>
  • Zhou H et al.Leukemia inhibitory factor promotes extracellular matrix synthesis in degenerative nucleus pulposus cells via MAPK-ERK1/2 signaling pathway.(2018)Biochem Biophys Res Commun;507(1-4):253-2Read more>>
  • Zhao R et al. Interleukin-1 receptor antagonist protein (IL-1Ra) and miR-140 overexpression via pNNS-conjugated chitosan-mediated gene transfer enhances the repair of full-thickness cartilage defects in a rabbit model. Bone Joint Res. 2019 Apr 2;8(3):165-178.Read more>>
  • Wang Y et al. Ryanodine receptor 1 mediated dexamethasone-induced chondrodysplasia in fetal rats. Biochim Biophys Acta Mol Cell Res. 2020 Oct;1867(10):118791.Read more>>
  • Kai Zhao. et al. Rhizoma drynariae total flavonoids inhibit the inflammatory response and matrix degeneration via MAPK pathway in a rat degenerative cervical intervertebral disc model. Biomed Pharmacother. 2021 Jun;138:111466Read more>>
  • J. Song. et al. Proteomics analysis of hip articular cartilage identifies differentially expressed proteins associated with osteonecrosis of the femoral head. Osteoarthr Cartilage. 2021 ApRead more>>
  • Xuejun Dai. et al. Comparison of the differentiation abilities of bone marrow?derived mesenchymal stem cells and adipose?derived mesenchymal stem cells toward nucleus pulposus?like cells in three?dimensional culture. Exp Ther Med. 2021 Sep;22(3):1-9Read more>>
  • Wenhui Hu. et al. Farnesoid X receptor agonist attenuates subchondral bone osteoclast fusion and osteochondral pathologies of osteoarthritis via suppressing JNK1/2/NFATc1 pathway. Faseb J. 2022 Apr;36(4):e22243Read more>>
  • Cheng, Baixiang. et al. Distinctive Roles of Wnt Signaling in Chondrogenic Differentiation of BMSCs under Coupling of Pressure and Platelet-Rich Fibrin. Tissue Engineering and Regenerative Medicine. 2022 Apr;:1-15Read more>>
  • Donglei Liu. et al. Effect of viscoelastic properties of cellulose nanocrystal/collagen hydrogels on chondrocyte behaviors. FRONT BIOENG BIOTECH. 2022; 10: 959409Read more>>
  • Changli Zhang. et al. PHLPP1 deficiency protects against age-related intervertebral disc degeneration. JOR SPINE. 2022 Sep;:e1224Read more>>
  • Tingting Xia. et al. Gardenoside ameliorates inflammation and inhibits ECM degradation in IL-1-treated rat chondrocytes via suppressing NF-B signaling pathways. BIOCHEM BIOPH RES CO. 2022 DecRead more>>
  • Flix Renaudin. et al. NADPH oxidase 4 deficiency attenuates experimental osteoarthritis in mice. RMD OPEN. 2023 Feb;9(1):e002856Read more>>
  • Haoran Wang. et al. CKLF1 interference alleviates IL?1?induced inflammation, apoptosis and degradation of the extracellular matrix in chondrocytes via CCR5. EXP THER MED. 2023 Jun;25(6):1-11Read more>>
  • Xiupeng Zhong. et al. Biomimetic Pericellular Microniche Inspired by Chondron enhances the Cartilage Matrix Accumulation. MACROMOL CHEM PHYS. 2023 Aug;:2300136Read more>>
  • Jintao Li. et al. Controlled-release hydrogel loaded with magnesium-based nanoflowers synergize immunomodulation and cartilage regeneration in tendon-bone healing. BIOACT MATER. 2024 Jun;36:62Read more>>
  • Xiaofeng Chen. et al. Exosomal U2AF2 derived from human bone marrow mesenchymal stem cells attenuates the intervertebral disc degeneration through circ_0036763/miR-583/ACAN axis. REGEN THER. 2024 Mar;25:344Read more>>
  • Liang Wang. et al. Myrislignan ameliorates the progression of osteoarthritis: An in vitro and in vivo study. INT IMMUNOPHARMACOL. 2024 Oct;140:112887Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat synovial tissue labeled with Anti-Aggrecan1 Polyclonal Antibody, Unconjugated (bs-1223R) followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-Aggrecan1 Polyclonal Antibody, Unconjugated (bs-1223R) at 1:200 followed by conjugation to the secondary antibody and DAB staining