Host: Rabbit
Target Protein: GRINA
Immunogen Range: 121-220/371
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2907
Swiss Prot: Q7Z429
Source: KLH conjugated synthetic peptide derived from human GRINA
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 41
Cross Reactive Species:
Human
Rat
Predicted Cross Reactive Species:
Mouse
Dog
Cow
Sheep
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \tGRINA Polyclonal Antibody, Unconjugated (bs-12098R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining
Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Rat Liver tissue Lysates; Lane 4: Rat Kidney tissue Lysates probed with GRINA Polyclonal Antibody, Unconjugated (bs-12098R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.