bs-1205R-RBITC [Conjugated Primary Antibody]
ROCK2 Polyclonal Antibody, RBITC Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ROCK2

Immunogen Range: 1001-1300/1388


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9475

Swiss Prot: O75116

Source: KLH conjugated synthetic peptide derived from human ROCK2

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP3, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p9RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

Conjugation: RBITC

Excitation/ Emission: 540nm/625nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 161


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Nan, et al. "Vascular endothelial growth factor stimulates endothelial differentiation from mesenchymal stem cells via Rho/myocardin-related transcription factor-A signaling pathway." The International Journal of Biochemistry & Cell Biology (2013).Read more>>
  • He, Hongpeng, et al. "Transcription of HOTAIR is regulated by RhoC-MRTF-A-SRF signaling pathway in human breast cancer cells." Cellular Signalling (2017).Read more>>
  • Zhu et al. Design and evaluation of EphrinA1 mutants with cerebral protective effect. (2017) Sci.Re. 7:1881Read more>>
  • Schwarz et al. Ccdc181 is a microtubule-binding protein that interacts with Hook1 in haploid male germ cells and localizes to the sperm tail and motile cilia. (2017) Eur.J.Cell.Bio. 96:276-288Read more>>
  • Rothschild et al. ROCK-1 mediates diabetes-induced retinal pigment epithelial and endothelial cell blebbing: Contribution to diabetic retinopathy. (2017) Sci.Rep. 7:8834Read more>>
  • Gui-Long Zhang. et al. Effect of conditioned medium from neural stem cells on glioma progression and its protein expression profile analysis. World J Stem Cells. 2020 Nov 26; 12(11): 1396C1409Read more>>
  • Yijie Liu. et al. Electroacupuncture improves retinal function in myopia Guinea pigs probably via inhibition of the RhoA/ROCK2 signaling pathway. HELIYON. 2024 Aug;1Read more>>
VALIDATION IMAGES