Host: Rabbit
Target Protein: CHRM4/mAChR M4
Immunogen Range: 1-31/479
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1132
Swiss Prot: P08173
Source: KLH conjugated synthetic peptide derived from human CHRM4/mAChR M4
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 53
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Tong L et al. Effects of topical pilocarpine on ocular growth and refractive development in rabbits. Eur J Ophthalmol. 2020 Jun 11;1120672120934962.Read more>>
A431 (Positive) and Huvec (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CHRM4/mAChR M4 Antibody (bs-11998R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
A431 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CHRM4/mAChR M4 Polyclonal Antibody(bs-11998R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).