bs-1196R [Primary Antibody]
F2RL3 (PAR4) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: F2RL3 (PAR4)

Immunogen Range: 320-343/385


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9002

Swiss Prot: Q96RI0

Source: KLH conjugated synthetic peptide derived from human PAR4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 41


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Oláh, Zita, et al. "Proteomic Analysis of Cerebrospinal Fluid in Alzheimers Disease: Wanted Dead or Alive." Journal of Alzheimers Disease (2014).Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti PAR4 Polyclonal Antibody, Unconjugated (bs-1196R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat pancreas labeled with Rabbit Anti PAR4 Polyclonal Antibody, Unconjugated (bs-1196R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Rat brain lysates probed with Anti PAR4 Polyclonal Antibody, Unconjugated (bs-1196R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 41kD. Observed band size:37kD, 44kD.\n


Lane 1: Mouse Lung tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Rat Lung tissue Lysates; Lane 4: Rat Thyroid gland tissue Lysates; Lane 5: Rat Testis tissue Lysates; Lane 6: Human K562 cell Lysates; Lane 7: Human THP-1 cell Lysates; Lane 8: Human Hela cell Lysates; Lane 9: Human Huvec cell Lysates probed with Proteinase-activated receptor 4/PAR4 Polyclonal Antibody, Unconjugated (bs-1196R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.