bs-1195R [Primary Antibody]
POMC Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: POMC

Immunogen Range: 237-267/267


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5443

Swiss Prot: P01189

Source: KLH conjugated synthetic peptide derived from human POMC

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

ACTH stimulates the adrenal glands to release cortisol. MSH (melanocyte-stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin and Met-enkephalin are endogenous opiates.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 4.5


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Cow
Sheep

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chao, Po-Kuan, et al. ʺEarly Systemic Granulocyte-Colony Stimulating Factor Treatment Attenuates Neuropathic Pain after Peripheral Nerve Injury.ʺ PloS one 7.8 (2012): e43680.Read more>>
  • Nakamoto, Kazuo, et al. ʺHypothalamic GPR40 Signaling Activated by Free Long Chain Fatty Acids Suppresses CFA-Induced Inflammatory Chronic Pain.ʺ PLOS ONE 8.12 (2013): e81563.Read more>>
  • Xie, Fang, et al. "Early Repeated Administration of CXCR4 Antagonist AMD3100 Dose-Dependently Improves Neuropathic Pain in Rats After L5 Spinal Nerve Ligation." Neurochemical Research (2016): 1-11.Read more>>
  • Tzu-Kai Lin. et al. Low-concentration imiquimod treatment promotes enhanced skin barrier functions through epidermal melanization reaction regulation. ENVIRON TOXICOL. 2024 MayRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse tumor labeled with Anti-β-endorphin Polyclonal Antibody, Unconjugated (bs-1195R) followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Mouse hypophysis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with POMC Polyclonal Antibody, Unconjugated (bs-1195R) at 1:400 overnight at 4°C, DAB staining.