Host: Rabbit
Target Protein: PAX2
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5076
Source: KLH conjugated synthetic peptide derived from human PAX2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Ji et al. Biological characterization of sheep kidney-derived mesenchymal stem cells. (2016) Exp.Ther.Me. 12:3963-3971Read more>>
- RUI-NING LIANG. et al. Kidney mesenchymal stem cells alleviate cisplatin-induced kidney injury and apoptosis in rats. TISSUE CELL. 2022 Dec;:101998Read more>>
Lane 1: mouse uterus lysates Lane 2: mouse brain lysates probed with Anti PAX2 Polyclonal Antibody, Unconjugated (bs-1187R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 46kD. Observed band size: 46kD.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PAX2 Antibody(bs-1187R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (blue).
Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebellum tissue lysates probed with PAX2 Polyclonal Antibody, Unconjugated (bs-1187R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.