bs-1184R [Primary Antibody]
TRAF6 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TRAF6

Immunogen Range: 441-530/530


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 22034

Swiss Prot: P70196

Source: KLH conjugated synthetic peptide derived from mouse TRAF6

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, IRAK1, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation (By similarity). Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 60


Cross Reactive Species: Human
Mouse
Rat
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Qi, Yonghao, et al. "Cinchonine induces apoptosis of HeLa and A549 cells through targeting TRAF6." Journal of Experimental & Clinical Cancer Research 36.1 (2017): 35.Read more>>
  • Li, Chengcheng, et al. "Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway." Scientific Reports 7 (2017).Read more>>
  • Wu Z et al. Co-infection of Mycoplasma gallisepticum and Escherichia coli Triggers Inflammatory Injury Involving the IL-17 Signaling Pathway. Front Microbiol. 2019 Nov 15;10:2615.Read more>>
  • Jin, Meiyuan. et al. MicroRNA-3935 promotes human trophoblast cell epithelial-mesenchymal transition through tumor necrosis factor receptor-associated factor 6/regulator of G protein signaling 2 axis. Reprod Biol Endocrin. 2021 Dec;19(1):1-15Read more>>
  • Yiheng Quan. et al. Bergenin ameliorates diabetic nephropathy in C57BL/6?J mice by TLR4/MyD88/NF-B signalling pathway regulation. TOXICOL APPL PHARM. 2023 Jul;:116633Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human oral squamous cell carcinoma labeled with Anti-TRAF6 Polyclonal Antibody, Unconjugated (bs-1184R) followed by conjugation to the secondary antibody and DAB staining


Lane 1: Rat Liver tissue lysates；Lane 2: Rat Kidney tissue lysates probed with TRAF6 Polyclonal Antibody, Unconjugated (bs-1184R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.