bs-11839R [Primary Antibody]
DNTNP/FAM53A Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: DNTNP/FAM53A

Immunogen Range: 255-340/398


Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from human DNTNP/FAM53A

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Dorsal neural-tube nuclear protein is a 398 amino acid nuclear protein. DNTNP is thought to play an important role in neural development by specifying dorsal cell fates within the neural tube. Widely expressed in the dorsal neural tube, DNTNP is most highly expressed in the dorsal regions of the midbrain, the hindbrain, the diencephalon, and the spinal neural tube and is expressed at lower levels in the branchial arches, the telencephalon, the heart, and somites of developing embryos. DNTNP is encoded by a gene located on chromosome 4 which contains many genes including the Huntingtin gene, which is found to encode an expanded glutamine tract in cases of Huntington's disease.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 43


Cross Reactive Species: Human
Dog

Predicted Cross Reactive Species: Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Dog testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DNTNP/FAM53A Polyclonal Antibody, Unconjugated (bs-11839R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DNTNP/FAM53A Polyclonal Antibody, Unconjugated (bs-11839R) at 1:200 overnight at 4°C, DAB staining.