Host: Rabbit
Target Protein: TMEM158
Immunogen Range: 188-260/300
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 25907
Swiss Prot: Q8WZ71
Source: KLH conjugated synthetic peptide derived from human TMEM158
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 28
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species:
Human
Cow
Pig
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TMEM158 Polyclonal Antibody, Unconjugated (bs-11770R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TMEM158 Polyclonal Antibody, Unconjugated (bs-11770R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAPI staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-TMEM158 Polyclonal Antibody, Unconjugated (bs-11770R) at 1:200 followed by conjugation to the secondary antibody and DAB staining