bs-1172R [Primary Antibody]
N-cadherin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: N-cadherin

Immunogen Range: 701-800/905


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1000

Swiss Prot: P19022

Source: KLH conjugated synthetic peptide derived from human N-cadherin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 100


Cross Reactive Species: Human
Mouse
Rat
Bovine
Zebrafish
Cow

Predicted Cross Reactive Species: Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu, Wen-bin, et al. "Aberrant methylation accounts for cell adhesion-related gene silencing during 3-methylcholanthrene and diethylnitrosamine induced multistep rat lung carcinogenesis associated with overexpression of DNA methyltransferases 1 and 3a." Toxicology and applied pharmacology 251.1 (2011): 70-78.Read more>>
  • Park, Honghyun, Doyun Kim, and Kuen Yong Lee. "Interaction‐tailored cell aggregates in alginate hydrogels for enhanced chondrogenic differentiation."Journal of Biomedical Materials Research Part A (2016).Read more>>
  • Yang, Wei, et al. "lncRNA H19 is involved in TGF-β1-induced epithelial to mesenchymal transition in bovine epithelial cells through PI3K/AKT Signaling Pathway." PeerJ 5 (2017): e3950.Read more>>
  • Fei et al. The number of polyploid giant cancer cells and epithelial-mesenchymal transition-related proteins are associated with invasion and metastasis in human breast cancer. (2015) J.Exp.Clin.Cancer.Res. 34:158Read more>>
  • Ning et al. Vascular endothelial growth factor receptor-1 activation promotes migration and invasion of breast cancer cells through epithelial-mesenchymal transition. (2013) PLoS.One. 8:e65217Read more>>
  • Wang et al. Kukoamine A inhibits human glioblastoma cell growth and migration through apoptosis induction and epithelial-mesenchymal transition attenuation. (2016) Sci.Rep. 6:36543Read more>>
  • Zhang, J., et al. “LGR5, a novel functional glioma stem cell marker, promotes EMT by activating the Wnt/β-catenin pathway and predicts poor survival of glioma patients.” Journal of Experimental & Clinical Cancer Research (2018); 37(1).Read more>>
  • Wu, DM., et al. “Long Non-Coding RNA LINC01260 Inhibits the Proliferation, Migration and Invasion of Spinal Cord Glioma Cells by Targeting CARD11 Via the NF-κB Signaling Pathway.” Cellular Physiology and Biochemistry (2018); 48(4): 1563-1578.Read more>>
  • Yu, AQ., et al. “TALEN-induced disruption of Nanog expression results in reduced proliferation, invasiveness and migration, increased chemosensitivity and reversal of EMT in HepG2 cells.” Oncology Reports (2015); 35(2): 1657-1663.Read more>>
  • Zhang J, et al. LGR5, a novel functional glioma stem cell marker, promotes EMT by activating the Wnt/β-catenin pathway and predicts poor survival of glioma patients.(2018) J. Exp. Clin. Cancer Res. Sep 12;37(1):225. Read more>>
  • Wu Y et al. MFAP5 promotes basal-like breast cancer progression by activating the EMT program.Cell Biosci. 2019 Mar 7;9:24. Read more>>
  • Zhou S et al. Helicobacter pylori infection promotes epithelial-to-mesenchymal transition of gastric cells by upregulating LAPTM4B. Biochem Biophys Res Commun. 2019 Jun 30;514(3):893-900.Read more>>
  • Ma T et al. MiR-940 inhibits migration and invasion of tongue squamous cell carcinoma via regulatingCXCR2/NF-κB system-mediated epithelial–mesenchymal transition. Naunyn Schmiedebergs Arch Pharmacol. 2019 Jun 18. Read more>>
  • Zhang D et al. Cell Membrane-Coated Porphyrin Metal-Organic Frameworks for Cancer Cell Targeting and O2-Evolving Photodynamic Therapy. ACS Appl Mater Interfaces. 2019 Oct 30;11(43):39594-39602.Read more>>
  • Liu H et al. Anti-tubulin agent vinorelbine inhibits metastasis of cancer cells by regulating epithelial-mesenchymal transition. Eur J Med Chem. 2020 Aug 15;200:112332.Read more>>
  • Jasmine Ercoliet al. KRIT1 as a possible new player in melanoma aggressiveness. Arch Biochem Biophys. 2020 Sep 30;691:108483.Read more>>
  • Mengmeng Niu. et al. Noncanonical TGF- signaling leads to FBXO3-mediated degradation of Np63 promoting breast cancer metastasis and poor clinical prognosis. Plos Biol. 2021 Feb;19(2):e3001113Read more>>
  • Xue Wang. et al. Engineered liposomes targeting the gutCCNS Axis for comprehensive therapy of spinal cord injury. J Control Release. 2021 Mar;331:39Read more>>
  • Francesca Salamanna. et al. Development and characterization of a novel human 3D model of bone metastasis from breast carcinoma in vitro cultured. Bone. 2021 Feb;143:115773Read more>>
  • Yu Guo. et al. RepSox effectively promotes the induced differentiation of sheep fibroblasts into adipocytes via the inhibition of the TGF?1/Smad pathway. Int J Mol Med. 2021 Aug;48(2):1-13Read more>>
  • Xingyi Xu. et al. A Honeycomb-Like Bismuth/Manganese Oxide Nanoparticle with Mutual Reinforcement of Internal and External Response for Triple-Negative Breast Cancer Targeted Therapy. 2021 Jul 23Read more>>
  • otao Pan. et al. Vorinostat targets UBE2C to reverse epithelial-mesenchymal transition and control cervical cancer growth through the ubiquitination pathway. Eur J Pharmacol. 2021 Oct;908:174399Read more>>
  • Ling Xie. et al. Suppression of GOLM1 by EGCG through HGF/HGFR/AKT/GSK-3/-catenin/c-Myc signaling pathway inhibits cell migration of MDA-MB-231. Food Chem Toxicol. 2021 Nov;157:112574Read more>>
  • Zheng Wu. et al. FOXD3 suppresses epithelialCmesenchymal transition through direct transcriptional promotion of SMAD7 in esophageal squamous cell carcinoma. 2021 Sep 22Read more>>
  • Zhao, Boyuan. et al. Suspension state and shear stress enhance breast tumor cells EMT through YAP by microRNA-29b. 2021 Oct 07Read more>>
  • Yuhua Li. et al. Bolbostemma paniculatum (Maxim.) Franquet extract suppresses the development of colorectal cancer through downregulation of PI3K/Akt pathway. J Ethnopharmacol. 2022 Apr;287:114937Read more>>
  • Haixiang Ding. et al. Ailanthone suppresses the activity of human colorectal cancer cells through the STAT3 signaling pathway. Int J Mol Med. 2022 Feb;49(2):1-11Read more>>
  • Yue, Huan. et al. LINC02154 promotes the proliferation and metastasis of hepatocellular carcinoma by enhancing SPC24 promoter activity and activating the PI3K-AKT signaling pathway. CELL ONCOL. 2022 May;:1-16Read more>>
  • Qi Rao. et al. Effects of dihydroartemisinin combined with cisplatin on proliferation, apoptosis and migration of HepG2 cells. ONCOL LETT. 2022 Aug;24(2):1-9Read more>>
  • Zheng, Bingxin. et al. Siglec-15-induced autophagy promotes invasion and metastasis of human osteosarcoma cells by activating the epithelialmesenchymal transition and Beclin-1/ATG14 pathway. CELL BIOSCI. 2022 Dec;12(1):1-15Read more>>
  • Cheng He. et al. Crosstalk of renal cell carcinoma cells and tumor-associated macrophages aggravates tumor progression by modulating muscleblind-like protein 2/B-cell lymphoma 2/beclin 1-mediated autophagy. CYTOTHERAPY. 2022 OcRead more>>
  • Guo, Zhi-chen. et al. Porphyromonas gingivalis promotes the progression of oral squamous cell carcinoma by activating the neutrophil chemotaxis in the tumour microenvironment. CANCER IMMUNOL IMMUN. 2022 Dec;:1-17Read more>>
  • Xu Yang. et al. Deoxynivalenol induces testicular ferroptosis by regulating the Nrf2/System Xc?/GPX4 axis. FOOD CHEM TOXICOL. 2023 May;175:11373Read more>>
  • Hao Cui. et al. Mena as a key enhancer factor of EMT to promote metastasis of human tongue squamous cell carcinoma. ORAL DIS. 2023 MayRead more>>
  • Fangyu Chai. et al. Identification of SLC2A3 as a prognostic indicator correlated with the NF-B/EMT axis and immune response in head and neck squamous cell carcinoma. CHANNELS. 2023;17(1):Article: 2208928Read more>>
  • Wu Kunzhe. et al. Co-delivery of curcumin and si-STAT3 with a bioinspired tumor homing for polydopamine nanoparticles for synergistic osteosarcoma therapy. CANCER NANOTECHNOL. 2023 Dec;14(1):1-21Read more>>
  • Xiang Lei. et al. Role of Cyclin D1b in Inducing Macrophages Toward a Tumor-associated Macrophage-like Phenotype in Murine Breast Cancer. CURR MED SCI. 2023 Jun;:1-13Read more>>
  • Zhang Jin. et al. ITGA2 as a prognostic factor of glioma promotes GSCs invasion and EMT by activating STAT3 phosphorylation. CARCINOGENESIS. 2023 DecRead more>>
  • Tamaki Naganuma. et al. Selective inhibition of partial EMT-induced tumour cell growth by cerium valence states of extracellular ceria nanoparticles for anticancer treatment. COLLOID SURFACE B. 2024 Apr;236:113794Read more>>
  • Dong Danhong. et al. YTHDC1 promotes the malignant progression of gastric cancer by promoting ROD1 translocation to the nucleus. CELL BIOL TOXICOL. 2024 Dec;40(1):1-15Read more>>
  • Yuan Zhao. et al. Dextran sulfate inhibits proliferation and metastasis of human gastric cancer cells via miR-34c-5p. HELIYON. 2024 Aug;1Read more>>
  • Xiaochuan Wang. et al. 5-aminolevulinic acid photodynamic therapy inhibits the viability, invasion, and migration of cervical cancer SiHa cells by regulating the miR-152-3p/JAK1/STAT1 axis. PHOTODIAGN PHOTODYN. 2024 Jul;:104283Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining


Formalin-fixed and paraffin embedded rat brain labeled with Anti-CDH2/N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin-embedded rat brain labeled with Anti-CDH2/N-cadherin Polyclonal Antibody, Unconjugated(bs-1172R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C.


Mouse breast cancer lysates probed with Anti-N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.