bs-1166R-Biotin [Conjugated Primary Antibody]
ROCK1 Polyclonal Antibody, Biotin Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ROCK1

Immunogen Range: 1001-1200/1354


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6093

Swiss Prot: Q13464

Source: KLH conjugated synthetic peptide derived from human ROCK1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Predicted Molecular Weight: 158


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Nan, et al. "Vascular endothelial growth factor stimulates endothelial differentiation from mesenchymal stem cells via Rho/myocardin-related transcription factor-A signaling pathway." The International Journal of Biochemistry & Cell Biology (2013).Read more>>
  • Gong, Tao, et al. "Dynamically tunable polymer microwells for directing mesenchymal stem cells differentiation into osteogenesis." Journal of Materials Chemistry B (2015).Read more>>
  • Pan ZN et al. RAB8A GTPase regulates spindle migration and Golgi apparatus distribution via ROCK-mediated actin assembly in mouse oocyte meiosis.(2018) Biol Reprod. Read more>>
  • Jiarong Yanget al. Effects of Different Doses of Calcium on the Mitochondrial Apoptotic Pathway and Rho/ROCK Signaling Pathway in the Bone of Fluorosis Rats. Biol Trace Elem Res. 2020 Jul 25.Read more>>
  • Xiaoli Gao. et al. Epigenetic upregulation of ssc-miR-124a following treatment with Clostridium perfringens beta2-toxin attenuates both apoptosis and inflammation in intestinal porcine epithelial cells. Arch Biochem Biophys. 2021 Apr;701:108806Read more>>
  • ianca Lourdes FREHNER. et al. Implications of the RhoA / Rho associated kinase pathway and leptin in primary uterine inertia in the dog. 2021 Mar 21Read more>>
  • Yuan-Jing Zou. et al. RAB14 GTPase is essential for actin-based asymmetric division during mouse oocyte maturation. 2021 Jul 29Read more>>
  • Nuo Heng. et al. RhoA improves cryopreservation of rooster sperm through the Rho/RhoA-associated Kinase/cofilin pathway. POULTRY SCIENCE. 2022 Jul;:102051Read more>>
VALIDATION IMAGES