bs-1161R [Primary Antibody]
Metabotropic glutamate receptor 2 Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Metabotropic glutamate receptor 2

Immunogen Range: 801-872/872


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2912

Swiss Prot: Q14416

Source: KLH conjugated synthetic peptide derived from human GRM2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. May mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 96


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Cow
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-GRM2/GLUR2 Polyclonal Antibody, Unconjugated (bs-1161R) at 1:300 followed by conjugation to the secondary antibody and DAB staining\n


Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Human SH-SY5Y cell lysates probed with GRM2 /Metabotropic glutamate receptor 2 Polyclonal Antibody, Unconjugated (bs-1161R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.