bs-1158R-A750
[Conjugated Primary Antibody]
AGEs Polyclonal Antibody, ALEXA FLUOR® 750 Conjugated
DATASHEET
Host: Rabbit
Target Protein: AGEs
Specificity: Reacts with AGEs.
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 280717
Swiss Prot: P02769
Source: AGEs
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Advanced Glycation End products (AGEs) are the result of a chain of chemical reactions after an initial glycation reaction. The intermediate products are known, variously, as Amadori, Schiff base and Maillard products, named after the researchers who first described them. (The literature is inconsistent in applying these terms. For example, Maillard reaction products are sometimes considered intermediates and sometimes end products.) Side products generated in intermediate steps may be oxidizing agents (such as hydrogen peroxide), or not (such as beta amyloid proteins). "Glycosylation" is sometimes used for "glycation" in the literature, usually as 'non-enzymatic glycosylation. The AGE-modified BSA was produced by reacting BSA with glycolaldehyde under sterile conditions followed by extensive dialysis and purification steps.
Conjugation: ALEXA FLUOR® 750
Excitation/ Emission: 749nm/775nm
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Kim DY et al. Eucalyptol Inhibits Advanced Glycation End Products‐Induced Disruption of Podocyte Slit Junctions by Suppressing Rage‐Erk‐C‐Myc Signaling Pathway.Mol Nutr Food Res. 2018 Oct;62(19):e1800302.Read more>>
- Ye, Ruidong, et al. "Ginsenoside Rd attenuates early oxidative damage and sequential inflammatory response after transient focal ischemia in rats." Neurochemistry international 58.3 (2011): 391-398.Read more>>
- Lu, Lin, et al. "Effects of atorvastatin on progression of diabetic nephropathy and local RAGE and soluble RAGE expressions in rats." Journal of Zhejiang University SCIENCE B 12.8 (2011): 652-659.Read more>>
- Tanaka M et al. Effects of combined treatment with blood flow restriction and low intensity electrical stimulation on diabetes mellitus-associated muscle atrophy in rats.J Diabetes. (2018) Sep 18Read more>>
- Oh H et al. Asaronic Acid Attenuates Macrophage Activation toward M1 Phenotype through Inhibition of NF-κB Pathway and JAK-STAT Signaling in Glucose-Loaded Murine Macrophages. J Agric Food Chem. 2019 Aug 26. Read more>>
- Kim DY et al. Eucalyptol Inhibits Advanced Glycation End Products‐Induced Disruption of Podocyte Slit Junctions by Suppressing Rage‐Erk‐C‐Myc Signaling Pathway.Mol Nutr Food Res. 2018 Oct;62(19):e1800302.Read more>>
- Eun-Jung Leeet al. Coumarin Ameliorates Impaired Bone Turnover by Inhibiting the Formation of Advanced Glycation End Products in Diabetic Osteoblasts and Osteoclasts. Biomolecules. 2020 Jul 15;10(7):1052.Read more>>
- Hee-Weon Lee. et al. Glyoxal-Lysine Dimer, an Advanced Glycation End Product, Induces Oxidative Damage and Inflammatory Response by Interacting with RAGE. Antioxidants-Basel. 2021 Sep;10(9):1486Read more>>
- Wanyi Zhou. et al. Glucose and MMP-9 dual-responsive hydrogel with temperature sensitive self-adaptive shape and controlled drug release accelerates diabetic wound healing. Bioact Mater. 2022 JaRead more>>
- Wei-Yu Yeh. et al. Functional chicken-liver hydrolysates ameliorate insulin resistance and cognitive decline in streptozotocin-induced diabetic mice. Poultry Sci. 2022 Mar;:101887Read more>>
- Zhuozhou Hu. et al. Acyclovir alleviates insulin resistance via activating PKM1 in diabetic mice. LIFE SCI. 2022 Sep;304:120725Read more>>
- Haiyan Zhou. et al. Glycation of Tie-2 Inhibits Angiopoietin-1 Signaling Activation and Angiopoietin-1-Induced Angiogenesis. INT J MOL SCI. 2022 Jan;23(13):7137Read more>>
- Dedert Cass. et al. Progranulin Preserves Autophagy Flux and Mitochondrial Function in Rat Cortical Neurons Under High Glucose Stress. FRONT CELL NEUROSCI. 2022 Jul;0:354Read more>>
- Andressa S. Sousa. et al. Evaluation of coronary function in female rats with severe type 1 diabetes: Effects of combined treatment with insulin and pyridoxamine. MICROVASC RES. 2022 Dec;:104474Read more>>
- Zou Ting-feng. et al. Fisetin treatment alleviates kidney injury in mice with diabetes-exacerbated atherosclerosis through inhibiting CD36/fibrosis pathway. ACTA PHARMACOL SIN. 2023 May;:1-1Read more>>
- Juanjuan Duan. et al. Benzo[a]pyrene and a high-fat diet induce aortic injury and promote low-density lipoprotein accumulation in the endothelium. ECOTOX ENVIRON SAFE. 2023 Jul;259:115011Read more>>
- Yang Sheng. et al. Advanced glycation end products promote meniscal calcification by activating the mTOR-ATF4 positive feedback loop. EXP MOL MED. 2024 Mar;:1-16Read more>>
- Inoue Hidenori. et al. Association between Moraxella keratitis and advanced glycation end products. SCI REP-UK. 2024 Apr;14(1):1-9Read more>>
- Yufang Ma. et al. Apigenin and baicalein ameliorate thoracic aortic structural deterioration and cognitive deficit via inhibiting AGEs/RAGE/NF-B pathway in D-galactose-induced aging rats. EUR J PHARMACOL. 2024 Aug;976:17666Read more>>
- Yi Yang. et al. Osteoking prevents bone loss and enhances osteoblastic bone formation by modulating the AGEs/IGF-1/-catenin/OPG pathway in type 2 diabetic db/db mice. CELL BIOL INT. 2024 JuRead more>>
- Zhang Xiu-Meng. et al. Piperazine ferulate inhibits diabetic nephropathy by suppressing AGE/RAGE-mediated inflammatory signaling in rats and podocytes. FRONT PHARMACOL. 2024 Aug;15Read more>>
VALIDATION IMAGES