bs-1158R
[Primary Antibody]
AGEs Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: AGEs
Specificity: Reacts with AGEs.
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 280717
Swiss Prot: P02769
Source: AGEs
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Advanced Glycation End products (AGEs) are the result of a chain of chemical reactions after an initial glycation reaction. The intermediate products are known, variously, as Amadori, Schiff base and Maillard products, named after the researchers who first described them. (The literature is inconsistent in applying these terms. For example, Maillard reaction products are sometimes considered intermediates and sometimes end products.) Side products generated in intermediate steps may be oxidizing agents (such as hydrogen peroxide), or not (such as beta amyloid proteins). "Glycosylation" is sometimes used for "glycation" in the literature, usually as 'non-enzymatic glycosylation. The AGE-modified BSA was produced by reacting BSA with glycolaldehyde under sterile conditions followed by extensive dialysis and purification steps.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Kim DY et al. Eucalyptol Inhibits Advanced Glycation End Products‐Induced Disruption of Podocyte Slit Junctions by Suppressing Rage‐Erk‐C‐Myc Signaling Pathway.Mol Nutr Food Res. 2018 Oct;62(19):e1800302.Read more>>
- Ye, Ruidong, et al. "Ginsenoside Rd attenuates early oxidative damage and sequential inflammatory response after transient focal ischemia in rats." Neurochemistry international 58.3 (2011): 391-398.Read more>>
- Lu, Lin, et al. "Effects of atorvastatin on progression of diabetic nephropathy and local RAGE and soluble RAGE expressions in rats." Journal of Zhejiang University SCIENCE B 12.8 (2011): 652-659.Read more>>
- Tanaka M et al. Effects of combined treatment with blood flow restriction and low intensity electrical stimulation on diabetes mellitus-associated muscle atrophy in rats.J Diabetes. (2018) Sep 18Read more>>
- Oh H et al. Asaronic Acid Attenuates Macrophage Activation toward M1 Phenotype through Inhibition of NF-κB Pathway and JAK-STAT Signaling in Glucose-Loaded Murine Macrophages. J Agric Food Chem. 2019 Aug 26. Read more>>
- Kim DY et al. Eucalyptol Inhibits Advanced Glycation End Products‐Induced Disruption of Podocyte Slit Junctions by Suppressing Rage‐Erk‐C‐Myc Signaling Pathway.Mol Nutr Food Res. 2018 Oct;62(19):e1800302.Read more>>
- Eun-Jung Leeet al. Coumarin Ameliorates Impaired Bone Turnover by Inhibiting the Formation of Advanced Glycation End Products in Diabetic Osteoblasts and Osteoclasts. Biomolecules. 2020 Jul 15;10(7):1052.Read more>>
- Hee-Weon Lee. et al. Glyoxal-Lysine Dimer, an Advanced Glycation End Product, Induces Oxidative Damage and Inflammatory Response by Interacting with RAGE. Antioxidants-Basel. 2021 Sep;10(9):1486Read more>>
- Wanyi Zhou. et al. Glucose and MMP-9 dual-responsive hydrogel with temperature sensitive self-adaptive shape and controlled drug release accelerates diabetic wound healing. Bioact Mater. 2022 JaRead more>>
- Wei-Yu Yeh. et al. Functional chicken-liver hydrolysates ameliorate insulin resistance and cognitive decline in streptozotocin-induced diabetic mice. Poultry Sci. 2022 Mar;:101887Read more>>
- Zhuozhou Hu. et al. Acyclovir alleviates insulin resistance via activating PKM1 in diabetic mice. LIFE SCI. 2022 Sep;304:120725Read more>>
- Haiyan Zhou. et al. Glycation of Tie-2 Inhibits Angiopoietin-1 Signaling Activation and Angiopoietin-1-Induced Angiogenesis. INT J MOL SCI. 2022 Jan;23(13):7137Read more>>
- Dedert Cass. et al. Progranulin Preserves Autophagy Flux and Mitochondrial Function in Rat Cortical Neurons Under High Glucose Stress. FRONT CELL NEUROSCI. 2022 Jul;0:354Read more>>
- Andressa S. Sousa. et al. Evaluation of coronary function in female rats with severe type 1 diabetes: Effects of combined treatment with insulin and pyridoxamine. MICROVASC RES. 2022 Dec;:104474Read more>>
- Zou Ting-feng. et al. Fisetin treatment alleviates kidney injury in mice with diabetes-exacerbated atherosclerosis through inhibiting CD36/fibrosis pathway. ACTA PHARMACOL SIN. 2023 May;:1-1Read more>>
- Juanjuan Duan. et al. Benzo[a]pyrene and a high-fat diet induce aortic injury and promote low-density lipoprotein accumulation in the endothelium. ECOTOX ENVIRON SAFE. 2023 Jul;259:115011Read more>>
- Yang Sheng. et al. Advanced glycation end products promote meniscal calcification by activating the mTOR-ATF4 positive feedback loop. EXP MOL MED. 2024 Mar;:1-16Read more>>
- Inoue Hidenori. et al. Association between Moraxella keratitis and advanced glycation end products. SCI REP-UK. 2024 Apr;14(1):1-9Read more>>
- Yufang Ma. et al. Apigenin and baicalein ameliorate thoracic aortic structural deterioration and cognitive deficit via inhibiting AGEs/RAGE/NF-B pathway in D-galactose-induced aging rats. EUR J PHARMACOL. 2024 Aug;976:17666Read more>>
- Yi Yang. et al. Osteoking prevents bone loss and enhances osteoblastic bone formation by modulating the AGEs/IGF-1/-catenin/OPG pathway in type 2 diabetic db/db mice. CELL BIOL INT. 2024 JuRead more>>
- Zhang Xiu-Meng. et al. Piperazine ferulate inhibits diabetic nephropathy by suppressing AGE/RAGE-mediated inflammatory signaling in rats and podocytes. FRONT PHARMACOL. 2024 Aug;15Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with AGEs Polyclonal Antibody, Unconjugated (bs-1158R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with AGEs Antibody(bs-1158R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Mouse Large intestine tissue lysates; Lane 4: Rat Testis tissue lysates ; Lane 5:Rat Lung tissue lysates ; Lane 6: Rat Large intestine tissue lysates; Lane 7: Human U87MG cell lysates probed with AGEs Polyclonal Antibody, Unconjugated (bs-1158R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with AGEs polyclonal antibody, unconjugated (bs-1158R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with AGEs polyclonal antibody, unconjugated (bs-1158R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.