DATASHEET
Host:
Rabbit
Target Protein:
Bid
Immunogen Range:
102-195/195
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
12122
Swiss Prot:
P70444
Source:
KLH conjugated synthetic peptide derived from mouse Bid
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Induces caspases and apoptosis. Counters the protective effect of Bcl-2. The major proteolytic product p15 BID allows the release of cytochrome c.
PRODUCT SPECIFIC PUBLICATIONS
- Fan et al. Resveratrol induces autophagy-dependent apoptosis in HL-60 cells. (2018) BMC.Cancer. 18:581Read more>>
- Guang\zhao Huang. et al. Screening and identification of autophagy\related biomarkers for oral squamous cell carcinoma (OSCC) via integrated bioinformatics analysis. 2021 Apr 09Read more>>
- Tiantian Qin. et al. Luteolin combined with low-dose paclitaxel synergistically inhibits epithelialCmesenchymal transition and induces cell apoptosis on esophageal carcinoma in vitro and in vivo. 2021 Sep 07Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded mouse brain labeled with Anti-Bid Polyclonal Antibody, Unconjugated (bs-1153R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bid) polyclonal Antibody, Unconjugated (bs-1153R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.