bs-11474R [Primary Antibody]
Repulsive Guidance Molecule B Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: RGMB/Repulsive Guidance Molecule B

Immunogen Range: 58-160/437


Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from human Repulsive Guidance Molecule B

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The repulsive guidance molecule (RGM) family of proteins are important in the guidance of growth cones of developing neurons. They are repulsive for a group of axons, those from the temporal half of the retina. RGM have been implicated in both axonal guidance and neural tube closure but as opposed to for ephrins, semaphorins, netrins and slits, no receptor mechanism for RGM activation has been defined. Dorsal root ganglion axons do not respond to RGM but neogenin (a netrin-binding protein which can function as an RGM receptor) expression can spur RGM responsiveness. The RGM proteins are attached to the membrane by a GPI-anchor. Two members of this family, RGMa and RGMb, are expressed in the nervous system. RGMc, also known as Hemojuvelin, is a part of the signaling pathway activating hepcidin and works together with hepcidin to restrict iron absorption in the gut. Defects in the gene encoding for RGMc causes the autosomal recessive disorder juvenile hemochromatosis (JH).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 40


Predicted Cross Reactive Species: Human
Mouse
Rat
Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Siu Yu A. Chow. et al. Human sensory neurons modulate melanocytes through secretion of RGMB. CELL REP. 2022 Sep;40:111366Read more>>
  • Guo Hua. et al. PD-L2 mediates tobacco smoking-induced recruitment of regulatory T cells via the RGMB/NFB/CCL20 cascade. CELL BIOL TOXICOL. 2024 Dec;40(1):1-21Read more>>
VALIDATION IMAGES

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Muscle tissue lysates; Lane 3: Mouse NIH/3T3 cell lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Stomach tissue lysates; Lane 6: Rat Muscle tissue lysates; Lane 7: Human A431 cell lysates probed with Repulsive Guidance Molecule B Polyclonal Antibody, Unconjugated (bs-11474R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.