Host: Rabbit
Target Protein: NG/Neurogranin
Immunogen Range: 30-78/78
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4900
Swiss Prot: Q92686
Source: KLH conjugated synthetic peptide derived from human NG/Neurogranin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 7.6
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species:
Human
Dog
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Neurogranin Polyclonal Antibody, Unconjugated(bs-11435R) 1:200, followed by conjugation to the secondary antibody and DAB.
Positive sample：Paraformaldehyde-fixed, paraffin embedded (rat brian, left); Negative sample：Paraformaldehyde-fixed, paraffin embedded (rat liver, right); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (NG/Neurogranin) at 37°C for 30min; Antibody incubation with (HDAC4) Polyclonal Antibody, Unconjugated (bs-11435R) at 1:200 overnight at 4°C, followed by secondary antibody and DAB staining.
Positive sample：Paraformaldehyde-fixed, paraffin embedded (mouse brain, left); Negative sample：Paraformaldehyde-fixed, paraffin embedded (mouse heart, right); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (NG/Neurogranin) at 37°C for 30min; Antibody incubation with (HDAC4) Polyclonal Antibody, Unconjugated (bs-11435R) at 1:200 overnight at 4°C, followed by secondary antibody and DAB staining.