bs-11410R [Primary Antibody]
Progestin Receptor Beta/MPRB Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Progestin Receptor Beta/MPRB

Immunogen Range: 251-300/354


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 85315

Source: KLH conjugated synthetic peptide derived from human Progestin Receptor Beta

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The steroid progesterone induces the resumption of maturation in oocytes via a nongenomic pathway through binding to a novel membrane progestin receptor (mPR). This pathway inhibits adenylyl cyclase and reduces intracellular cAMP, and also activates mitogen-activated protein kinase to effect signal transduction pathways. Five distinct groups, designated Alpha, Beta, Gamma, Delta, comprise the mPR gene family. mPR Alpha, also designated progestin and adipo Theta receptor family member VII (PAQR7), consists of an extracellular N-terminus, an intracellular C-terminus and seven transmembrane domains. mPR Alpha is expressed in ovary, testis, placenta, uterus and bladder. mPR Beta, or progestin and adipo Theta receptor family member VIII (PAQR8), consists of eight putative transmembrane regions and an intracellular N-terminus that contains a leucine-rich motif. mPR Beta is a 354 amino acid protein expressed in brain and spinal cord. Both mPR Alpha and mPR Beta may be G protein-coupled receptors and may be involved in oocyte maturation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:500-2000)
ELISA(1:5000-20000)

Predicted Molecular Weight: 40


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Kasubuchi, Mayu, et al. "Membrane progesterone receptor beta (mPRβ/Paqr8) promotes progesterone-dependent neurite outgrowth in PC12 neuronal cells via non-G protein-coupled receptor (GPCR) signaling." Scientific Reports 7 (2017).Read more>>
  • Gao XX et al. Effects of l-arginine on endometrial estrogen receptor α/β and progesterone receptor expression in nutrient-restricted sheep. Theriogenology. 2019 Jul 25;138:137-144.Read more>>
  • Thomas Topilko. et al. Edinger-Westphal peptidergic neurons enable maternal preparatory nesting. Neuron. 2022 FebRead more>>
VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Progestin Receptor Beta polyclonal antibody, unconjugated (bs-11410R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.