bs-11397R [Primary Antibody]
Arginase 2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Arginase 2

Immunogen Range: 181-290/354


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 384

Swiss Prot: P78540

Source: KLH conjugated synthetic peptide derived from human Arginase II

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to NO synthase. Since NO synthase is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase II plays a role in both male and female sexual arousal. It is therefore a potential target for the treatment of male and female sexual arousal disorders.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 36


Cross Reactive Species: Human
Mouse
Sheep

Predicted Cross Reactive Species: Rat
Dog
Cow
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zaytouni et al. Critical role for arginase 2 in obesity-associated pancreatic cancer. (2017) Nat.Commun. 8:242Read more>>
  • Liu Y et al. Isolation and characterization of ovine monocyte-derived macrophages from peripheral blood.(2018)Vet Immunol Immunopathol. Nov;205:83-92. Read more>>
  • Aihara Seishi. et al. Spermidine from arginine metabolism activates Nrf2 and inhibits kidney fibrosis. COMMUN BIOL. 2023 Jun;6(1):1-14Read more>>
  • Imazu Noriyuki. et al. Arginase 2 attenuates ulcerative colitis by antioxidant effects of spermidine. J GASTROENTEROL. 2024 May;:1-17Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti Arginase II Polyclonal Antibody, Unconjugated (bs-11397R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human kidney labeled with Anti-Arginase II Polyclonal Antibody, Unconjugated (bs-11397R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


DU145 Cell lysates; probed with Arginase II Polyclonal Antibody, unconjugated (bs-11397R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Arginase II) polyclonal Antibody, Unconjugated (bs-11397R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.