bs-11216R

DATASHEET

Host: Rabbit

Target Protein: Sprouty1

Immunogen Range: 221-319/319

Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from human Sprouty1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Members of the Sprouty family (Sprouty 1-4) are inducible negative regulators of growth factors that act through tyrosine kinase receptors. Mammalian Sprouty homologs share a well-conserved cysteine-rich C-terminal domain with their Drosophila counterparts. Both Sprouty 1 and 2 are anchored to membranes by palmitoylation, associate with caveolin-1 in perinuclear and vesicular structures and are phosphorylated on Serine residues. Upon stimulation, a subset is recruited to the leading edge of the plasma membrane. Sprouty 2 can associate with c-Cbl, a down regulator of RTK signaling, and inhibits the activities of several growth factors. Sprouty 2 also functions as a negative regulator of embryonic lung morphogenesis and growth. The well-conserved C-terminus of Sprouty contains two domains which are necessary for Sprouty 2 co-localization with microtubules and translocation to membrane ruffles. In addition, the C-terminus is required for the inhibition of cell migration and proliferation. In conclusion, members of Sprouty inhibit FGF and VEGF-mediated cell proliferation, suggesting that they may regulate angiogenesis in normal and disease processes.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 35

Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Cow
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.