bs-1117R [Primary Antibody]
GPR49/LGR5 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GPR49/LGR5

Immunogen Range: 810-907/907


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 14160

Swiss Prot: Q9Z1P4

Source: KLH conjugated synthetic peptide derived from mouse GPR49

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)

Predicted Molecular Weight: 98


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Su, Zhongwu, et al. "Ionizing radiation promotes advanced malignant traits in nasopharyngeal carcinoma via activation of epithelial-mesenchymal transition and the cancer stem cell phenotype." Oncology Reports (2016).Read more>>
  • Lai Wei. et al. Serotonin Deficiency is Associated with Delayed Gastric Emptying. Gastroenterology. 2021 MaRead more>>
  • Maiko Machida. et al. Methotrexate mediates the integrity of intestinal stem cells partly through nitric oxide-dependent Wnt/_-catenin signaling in methotrexate-induced rat ileal mucositis. J Pharmacol Sci. 2022 Mar;148:281Read more>>
  • Yan Ziqiao. et al. Therapeutic mechanism of Liangxue-Guyuan-Yishen decoction on intestinal stem cells and tight junction proteins in gastrointestinal acute radiation syndrome rats. J RADIAT RES. 2023 SepRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-GPR49/LGR5 Polyclonal Antibody, Unconjugated (bs-1117R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded mouse colon carcinoma labeled with Anti-GPR49/LGR5 Polyclonal Antibody, Unconjugated (bs-1117R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


MCF-7 cells probed with GPR49/LGR5 Polyclonal Antibody, unconjugated (bs-1117R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)