bs-11167R [Primary Antibody]
VGLUT1/ VGLUT2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: VGLUT1

Specificity: The immunogen shares 100% sequence similarity with VGLUT2.

Immunogen Range: 301-400/560


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 57030

Source: KLH conjugated synthetic peptide derived from human VGLUT1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. Involved in the regulation of retinal hyaloid vessel regression during postnatal development.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wan, Dong-Feng. et al. Late Exercise Preconditioning Regulates BNP Increasing to Assist the Cardioprotection via Up-Regulation of NPR-A and Down-Regulation of NPR-C in Rat Myocardium. Int J Pept Res Ther. 2022 Jan;28(1):1-11Read more>>
  • Canhong Wang. et al. Agarwood essential oil inhalation exerts antianxiety and antidepressant effects via the regulation of Glu/GABA system homeostasis. BIOMED REP. 2023 Feb;18(2):1-11Read more>>
  • Qian Fang. et al. YTHDF1 phase separation triggers the fate transition of spermatogonial stem cells by activating the IB-NF-B-CCND1 axis. CELL REP. 2023 Apr 14;42(4):112403Read more>>
  • Xiaoting Meng. et al. Electrical stimulation induced structural 3D human engineered neural tissue with well-developed neuronal network and functional connectivity. J NEURAL ENG. 2023 JulRead more>>
VALIDATION IMAGES

Mouse brain lysates probed with Rabbit Anti-VGLUT1 Polyclonal Antibody, Unconjugated (bs-11167R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody for 90 min at 37˚C.


Lane 1: Rat Cerebrum tissue lysates; Lane 2: Human SH-SY5Y cell lysates; Lane 3: Human MCF-7 cell lysates probed with VGLUT1/BNP1 Polyclonal Antibody, Unconjugated (bs-11167R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VGLUT1/BNP1 Polyclonal Antibody, Unconjugated (bs-11167R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VGLUT1/BNP1 Polyclonal Antibody, Unconjugated (bs-11167R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VGLUT1/BNP1 Polyclonal Antibody, Unconjugated (bs-11167R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.