bs-11164R-Cy5.5 [Conjugated Primary Antibody]
Sidekick 1 Polyclonal Antibody, Cy5.5 Conjugated
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Host: Rabbit

Target Protein: Sidekick 1

Immunogen Range: 1501-1600/2213


Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from human Sidekick 1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Cell adhesion molecules influence cell growth, differentiation, embryogenesis, immune response and cancer metastasis by networking information from the extracellular matrix to the cell. Sidekick-1 (SDK1) is a 2,213 amino acid single-pass membrane protein that functions as a cell adhesion molecule by guiding axonal terminals to specific synapses in developing neurons. Existing as three alternatively spliced isoforms, Sidekick-1 is expressed in retinal neurons and contains thirteen fibronectin type-III domains and six Ig-like C2-type (immunoglobulin-like) domains. Sidekick-1 expression is upregulated in glomeruli of patients with HIV-associated nephropathy, where it leads to podocyte dysfunction. The gene encoding Sidekick-1 maps to human chromosome 7p22.2 and murine chromosome 5 G2.

Conjugation: Cy5.5

Excitation/ Emission: 675nm/694nm

Size: 100ul

Concentration: 1ug/ul

Applications: FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 242


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Sheep
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

U2OS cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Sidekick 1 Polyclonal Antibody, Cy5.5 Conjugated (bs-11164R-Cy5.5) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).