bs-1115R
[Primary Antibody]
AMPK alpha-1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: AMPK alpha-1
Immunogen Range: 351-450/559
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5562
Swiss Prot: Q13131
Source: KLH conjugated synthetic peptide derived from human AMPK alpha 1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq].
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
Predicted Molecular Weight: 64
Cross Reactive Species:
Human
Mouse
Rat
Pig
Sheep
Predicted Cross Reactive Species:
Dog
Cow
Horse
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wang, Yandi, et al. "Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis." Toxicology and Applied Pharmacology (2016).Read more>>
- Zhao, Yong, et al. "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." Scientific Reports 6 (2016): 37884.Read more>>
- Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).Read more>>
- Wang et al. Protective effects of melatonin against nicotine-induced disorder of mouse early folliculogenesis. (2018) Aging.(Albany.NY). 10:463-480Read more>>
- Yao C et al. Data Mining and Validation of AMPK Pathway as a Novel Candidate Role Affecting Intramuscular Fat Content in Pigs. Animals (Basel). 2019 Apr 1;9(4). pii: E137. Read more>>
- Huang H et al. AMPK mediates the neurotoxicity of iron oxide nanoparticles retained in mitochondria or lysosomes. Metallomics. 2019 Jun 26.Read more>>
- Wang J et al. Novel anti-obesity peptide (RLLPH) derived from hazelnut (Corylus heterophylla Fisch) protein hydrolysates inhibits adipogenesis in 3T3-L1 adipocytes by regulating adipogenic transcription factors and adenosine monophosphate-activated protein kinase (AMPK) activation. J Biosci Bioeng. 2019 Oct 17. pii: S1389-1723(19)30741-8. Read more>>
- Yang Q et al. Hexavalent chromium induces mitochondrial dynamics disorder in rat liver by inhibiting AMPK/PGC-1 signaling pathway. Environ Pollut.2020 Oct;265(Pt A):114855.Read more>>
- Tang S et al. High ammonia exposure regulates lipid metabolism in the pig skeletal muscle via mTOR pathway. Sci Total Environ. 2020 Oct 20;740:139917.Read more>>
- Li T et al. Fufang-Zhenzhu-Tiaozhi capsule ameliorates rabbits iliac artery restenosis by regulating adiponectin signaling pathway. Biomed Pharmacother. 2020 Aug;128:110311.Read more>>
- Qing Zenget al. Exosomes Secreted From Bone Marrow Mesenchymal Stem Cells Attenuate Oxygen-Glucose Deprivation/Reoxygenation-Induced Pyroptosis in PC12 Cells by Promoting AMPK-Dependent Autophagic Flux. Front Cell Neurosci. 2020 Jul 17;14:182.Read more>>
- Weiyi Zhanget al. Cinnamaldehyde changes the dynamic balance of glucose metabolism by targeting ENO1. Life Sci. 2020 Oct 1;258:118151.Read more>>
- Wei C et al. Nanocolloids in drinking water increase the risk of obesity in mice by modulating gut microbesEnviron Int.2021 Jan;146:106302.Read more>>
- Ma Y et al. IRE1 and CaMKK pathways to reveal the mechanism involved in microcystin-LR-induced autophagy in Mouseovarian cellsFood Chem Toxicol.2021 Jan;147:111911.Read more>>
- Cui Z. et al. High Expression of miR-204 in Chicken Atrophic Ovaries Promotes Granulosa Cell Apoptosis and Inhibits Autophagy.. Front Cell Dev Biol. 2020 Nov;8:580072-580072Read more>>
- Dong Zhixia. et al. Adiponectin Inhibits NLRP3 Inflammasome Activation in Nonalcoholic Steatohepatitis via AMPK-JNK/ErK1/2-NFB/ROS Signaling Pathways. Front Med-Lausanne. 2020 Nov;7:705Read more>>
- Mengmeng Yu. et al. Taurine attenuates gossypol-induced apoptosis of C2C12 mouse myoblasts via the GPR87-AMPK/AKT signaling. Amino Acids. 2020 Sep;52(9):1285-1298Read more>>
- Feng Yanzhong. et al. Ulva prolifera Extract Alleviates Intestinal Oxidative Stress via Nrf2 Signaling in Weaned Piglets Challenged With Hydrogen Peroxide. Front Immunol. 2020 Oct;11:284Read more>>
- Tian\Yu Feng. et al. Rosmarinic acid improves boar sperm quality, antioxidant capacity and energy metabolism at 17C via AMPK activation. Reprod Domest Anim. 2020 Dec;55(12):1714-1724Read more>>
- Zhang-Jie Yang. et al. SIRT 3 was involved in Lycium barbarum seed oil protection testis from oxidative stress: in?vitro and in?vivo analyses. Pharm Biol. 2021;59(1):1314-1325Read more>>
- olin Cai. et al. LncEDCH1 improves mitochondrial function to reduce muscle atrophy by interacting with sarcoplasmic/endoplasmic reticulum calcium ATPase 2. Mol Ther-Nucl Acids. 2021 DecRead more>>
- Cai, Bolin. et al. circPTPN4 regulates myogenesis via the miR-499-3p/NAMPT axis. J Anim Sci Biotechno. 2022 Dec;13(1):1-15Read more>>
- Jie Gao. et al. Ferulic acid targets ACSL1 to ameliorate lipid metabolic disorders in db/db mice. J Funct Foods. 2022 Apr;91:105009Read more>>
- Jing Fan. et al. Chitosan Oligosaccharide Inhibits the Synthesis of Milk Fat in Bovine Mammary Epithelial Cells through AMPK-Mediated Downstream Signaling Pathway. ANIMALS. 2022 Jan;12(13):1692Read more>>
- Deng Disi. et al. Protective Effect of XinJiaCongRongTuSiZiWan on the Reproductive Toxicity of Female Rats Induced by Triptolide. EVID-BASED COMPL ALT. 2022;2022:3642349Read more>>
- Yu Wang. et al. Seabuckthorn Reverses High-Fat-Diet-Induced Obesity and Enhances Fat Browning via Activation of AMPK/SIRT1 Pathway. NUTRIENTS. 2022 Jan;14(14):2903Read more>>
- Rui Guo. et al. Ulinastatin attenuates spinal cord injury by targeting AMPK/NLRP3 signaling pathway. J CHEM NEUROANAT. 2022 Nov;125:102145Read more>>
- Xing Zhang. et al. Effect of Astragalus polysaccharides on the cryopreservation of goat semen. THERIOGENOLOGY. 2022 AugRead more>>
- Lifang Li. et al. Changes in the Expression of MIF and Other Key Enzymes of Energy Metabolism in the Myocardia of Broiler Chickens with Ascites Syndrome. ANIMALS. 2022 Jan;12(19):2488Read more>>
- Mingjuan Yang. et al. Rosmarinic acid potentiates and detoxifies tacrine in combination for Alzheimer's disease. PHYTOMEDICINE. 2022 Dec;:1546Read more>>
- Qidang Duan. et al. LOX-1 attenuates high glucose-induced autophagy via AMPK/HNF4 signaling in HLSECs. HELIYON. 2022 Dec;8:e12385Read more>>
- Jing Fan. et al. Syndecan-3 Coregulates Milk Fat Metabolism and Inflammatory Reactions in Bovine Mammary Epithelial Cells through AMPK/SIRT1 Signaling Pathway. INT J MOL SCI. 2023 Jan;24(7):6657Read more>>
- Wei Yu. et al. Glucose promotes cell growth and casein synthesis via ATF4/Nrf2-Sestrin2- AMPK-mTORC1 pathway in dairy cow mammary epithelial cells. ANIM BIOTECHNOL. 2023 Jul 12Read more>>
- W.J. Tu. et al. Osteocalcin activates lipophagy via the ADPN-AMPK/PPAR-mTOR signaling pathway in chicken embryonic hepatocyte. POULTRY SCIENCE. 2023 Nov;:103293Read more>>
- Yaqi Li. et al. Non-thermal plasma promotes boar sperm quality through increasing AMPK methylation. INT J BIOL MACROMOL. 2024 Feb;257:128768Read more>>
- Zhendong Zhu. et al. Resveratrol Improves the Frozen-Thawed Ram Sperm Quality. ANIMALS. 2023 Jan;13(24):3887Read more>>
- Xiu-Li Zhang. et al. MCT1-mediated transport of valeric acid promotes porcine preimplantation embryo development by improving mitochondrial function and inhibiting the autophagic AMPK-ULK1 pathway. THERIOGENOLOGY. 2024 Sep;225:152Read more>>
- Sun Xuri. et al. Exogenous NT-3 Promotes Phenotype Switch of Resident Macrophages and Improves Sciatic Nerve Injury through AMPK/NF-B Signaling Pathway. NEUROCHEM RES. 2024 Jun;:1-15Read more>>
- Li Zhipeng. et al. The role of quercetin in ameliorating bleomycin-induced pulmonary fibrosis: insights into autophagy and the SIRT1/AMPK signaling pathway. MOL BIOL REP. 2024 Dec;51(1):1-1Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Anti-AMPK alpha-1/PRKAA1 Polyclonal Antibody, Unconjugated (bs-1115R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse colon labeled with Anti-AMPK alpha-1/PRKAA1 Polyclonal Antibody, Unconjugated (bs-1115R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human kidney labeled with Anti-AMPK alpha-1 Polyclonal Antibody, Unconjugated (bs-1115R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Human Hela lysates probed with AMPK alpha 1 Polyclonal Antibody, Unconjugated (bs-1115R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
U937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with AMPK alpha-1 Polyclonal Antibody(bs-1115R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Mouse spleen cells probed with AMPK alpha-1 Polyclonal Antibody, unconjugated (bs-1115R) at 1:100 dilution for 30 minutes compared to control cells (purple), secondary only (blue) and isotype control (orange)
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with AMPK alpha 1 Polyclonal Antibody(bs-1115R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).