bs-11142R [Primary Antibody]
Rho B Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Rho B

Immunogen Range: 101-196/196


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 388

Source: KLH conjugated synthetic peptide derived from human Rho B

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The Rho proteins interact directly with protein kinases which are likely to serve as downstream effector targets of the activated GTPase. The Rho family of GTPases are involved in the regulation of the actin skeleton. Activation of Rho to the GTP bound state promotes formation of stress fibers and is required for the formation and maintenance of focal adhesions. Pathways stimulated by cytokines, hormones and various forms of stress utilize p21 proteins of the Rho family. Possible mediators for these responses include protein kinase such as p120PKN, and p150 ROKa/Rho-A kinase, myosin light chain (MLC) phosphatase and PIP 5-kinase.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 22


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Choi, Bunsoon, et al. "The Relevance of miRNA-21 in HSV-Induced Inflammation in a Mouse Model." International Journal of Molecular Sciences 16.4 (2015): 7413-7427.Read more>>
  • Jun Li. et al. WJ?MSCs intervention may relieve intrauterine adhesions in female rats via TGF?β1?mediated Rho/ROCK signaling inhibition. Mol Med Rep. 2021 Jan;23(1):1-1Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human pancreatic cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Rho B Polyclonal Antibody, Unconjugated (bs-11142R) at 1:400 overnight at 4°C, DAB staining.