DATASHEET
Host:
Rabbit
Target Protein:
Lamin B2
Immunogen Range:
61-160/600
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
84823
Swiss Prot:
Q03252
Source:
KLH conjugated synthetic peptide derived from human Lamin B2
Purification:
Purified by Protein A.
Storage Buffer:
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
A unique family of Cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, termed CED-3/ICE, functions as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Nuclear lamins are critical to maintaining the integrity of the nuclear envelope and cellular morphology as components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family.
Conjugation:
Biotin
Excitation/ Emission:
N/A
Size:
100ul
Concentration:
1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
68
Mouse
Predicted Cross Reactive Species:
Human
Rat
Cow
Sheep
For research use only. Not intended for diagnostic or therapeutic use.