bs-1097R [Primary Antibody]
PAX3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PAX3

Immunogen Range: 151-250/479


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 18505

Swiss Prot: P24610

Source: KLH conjugated synthetic peptide derived from mouse PAX3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcription factor that may regulate cell proliferation, migration and apoptosis. Involved in neural development and myogenesis.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 53


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Bonchak, Jonathan G., et al. "Targeting melanocyte and melanoma stem cells by 8-hydroxy-2-dipropylaminotetralin." Archives of Biochemistry and Biophysics (2014).Read more>>
  • Rooper, Lisa M., et al. "Biphenotypic Sinonasal Sarcoma: an Expanded Immunoprofile Including Consistent Nuclear Beta-Catenin Positivity and Absence of SOX10 Expression." Human Pathology (2016).Read more>>
  • Dong, Changsheng, et al. "Functional Role of Cyclin-Dependent Kinase 5 in the Regulation of Melanogenesis and Epidermal Structure." Scientific Reports (Nature Publisher Group) 7 (2017): 1.Read more>>
  • Li, Xinxin, et al. "Long-term thermal manipulation in the late incubation period can inhibit breast muscle development by activating endoplasmic reticulum stress in duck (Anasplatyrhynchos domestica)." Journal of thermal biology 70.Pt B (2017): 37.Read more>>
  • Yuanyuan Chen. et al. Single-Cell Characterization of the Frizzled 5 (Fz5) Mutant Mouse and Human Persistent Fetal Vasculature (PFV). INVEST OPHTH VIS SCI. 2023 Mar;64(3):8-8Read more>>
VALIDATION IMAGES

Lane 1: mouse embryo lysates Lane 2: human colon carcinoma lysates probed with Anti PAX3 Polyclonal Antibody, Unconjugated (bs-1097R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 53kD. Observed band size: 53kD


Formalin-fixed and paraffin embedded human esophageal carcinoma labeled with Anti-PAX3 Polyclonal Antibody, Unconjugated(bs-1097R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat brain labeled with Anti-PAX3 Polyclonal Antibody, Unconjugated(bs-1097R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Mouse Cerebellum tissue lysates; Lane 2: Mouse Muscle tissue lysates; Lane 3: Rat Cerebellum tissue lysates; Lane 4: Rat Muscle tissue lysates probed with PAX3 Polyclonal Antibody, Unconjugated (bs-1097R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.