bs-1092R
[Primary Antibody]
MRP2 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: MRP2
Immunogen Range: 485-615/1545
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1244
Swiss Prot: Q92887
Source: KLH conjugated synthetic peptide derived from human MRP2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 174
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Yuan P et al. WS2 Nanosheets at Noncytotoxic Concentrations Enhance the Cytotoxicity of Organic Pollutants by Disturbing the Plasma Membrane and Efflux Pumps. Environ Sci Technol. 2020 Feb 4;54(3):1698-1709.Read more>>
- Haruna Makita. et al. Xenografts derived from patients with head and neck cancer recapitulate patient tumour properties. Oncol Lett. 2021 May;21(5):1-1Read more>>
- Xin Fang. et al. The changes of MRP2 expression in three kinds of pulmonary inflammation models: the downregulation occurred in cigarette smoke extract (CSE) stimulation group and CSE plus LPS stimulation group, unchanged in LPS stimulation group. 2021 Mar 23Read more>>
- Shimeng Qiu. et al. An Efficient Method for the Differentiation of Human iPSC-Derived Endoderm toward Enterocytes and Hepatocytes. Cells-Basel. 2021 Apr;10(4):812Read more>>
- Xin Fang. et al. Cigarette smoke extract combined with LPS down-regulates the expression of MRP2 in chronic pulmonary inflammation may be related to FXR. Mol Immunol. 2021 Sep;137:174Read more>>
- Caijun Zhao. et al. Kynurenic acid protects against mastitis in mice by ameliorating inflammatory responses and enhancing blood-milk barrier integrity. Mol Immunol. 2021 Sep;137:134Read more>>
- Yuge Liao. et al. Inorganic mercury induces liver oxidative stress injury in quails by inhibiting Akt/Nrf2 signal pathway. INORG CHEM COMMUN. 2022 Aug;142:109603Read more>>
- Silvia Di Giacomo. et al. Sorafenib Chemosensitization by Caryophyllane Sesquiterpenes in Liver, Biliary, and Pancreatic Cancer Cells: The Role of STAT3/ABC Transporter Axis. PHARMACEUTICS. 2022 Jun;14(6):1264Read more>>
- Zipeng Gong. et al. Pharmacokinetic Differences of Wuji Pill Components in Normal and Chronic Visceral Hypersensitivity Irritable Bowel Syndrome Rats Attributable to Changes in Tight Junction and Transporters. FRONT PHARMACOL. 2022; 13: 948678Read more>>
- Tang Bo. et al. Gut microbiota alters host bile acid metabolism to contribute to intrahepatic cholestasis of pregnancy. NAT COMMUN. 2023 Mar;14(1):1-17Read more>>
- Yilin Sun. et al. Rhus chinensis Mill. fruits alleviate liver injury induced by isoniazid and rifampicin through regulating oxidative stress, apoptosis, and bile acid transport. J ETHNOPHARMACOL. 2023 Jun;310:116387Read more>>
- erezin Casey-Tyler. et al. Morphine pharmacokinetics and opioid transporter expression at the blood-retina barrier of male and female mice. FRONT PHARMACOL. 2023 Jun;14Read more>>
- Zahra Zakeri Khatir. et al. 4-{3-[(Pyridin-4-ylmethyl)amino]-[1,2,4]triazolo[4,3-b][1,2,4]triazin-6-yl}phenol: An improved anticancer agent in hepatocellular carcinoma and a selective MDR1/MRP modulator. ARCH PHARM. 2024 Mar;:e2300704Read more>>
- Arzu Kaya Kocdogan. et al. INVESTIGATION OF GST AND DRUG RESISTANCE PROTEIN EXPRESSIONS IN RELATION TO CHEMOTHERAPY IN BREAST CANCER. HEALTH PROBL CIVILIZ. 2024 May;18(1)Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human liver carcinoma labeled with Anti-MRP2 Polyclonal Antibody, Unconjugated (bs-1092R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin-embedded human liver carcinoma labeled with Anti-MRP2 Polyclonal Antibody, Unconjugated(bs-1092R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG,PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C.
Lane 1: Human HepG2 cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human Panc-1 cell lysates probed with MRP2 Polyclonal Antibody, Unconjugated (bs-1092R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cell lysates probed with ABCC2/MRP2 Polyclonal Antibody, Unconjugated (bs-1092R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse liver lysates probed with ABCC2/MRP2 Polyclonal Antibody, Unconjugated (bs-1092R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MRP2) Polyclonal Antibody, Unconjugated (bs-1092R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MRP2/ABCC2 Polyclonal Antibody, Unconjugated (bs-1092R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.