bs-1091R-Biotin [Conjugated Primary Antibody]
NADPH oxidase 4 Polyclonal Antibody, Biotin Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NADPH oxidase 4

Immunogen Range: 81-180/578


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 50490

Swiss Prot: Q9JHI8

Source: KLH conjugated synthetic peptide derived from mouse Nox-4

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB.

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IP(1-2ug)

Predicted Molecular Weight: 64


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ma Q et al. Non‐coenzyme role of vitamin B1 in RANKL‐induced osteoclastogenesis and ovariectomy induced osteoporosis. J Cell Biochem. 2020 Feb 26.Read more>>
  • Ulmasov et al. CLIC1 null mice demonstrate a role for CLIC1 in macrophage superoxide production and tissue injury. (2017) Physiol.Rep. 5 Read more>>
  • Wang R et al. Leonurine alleviates LPS-induced myocarditis through suppressing the NF-кB signaling pathway. Toxicology. 2019 Apr 18;422:1-13.Read more>>
  • Takashina Y et al. Sodium Citrate Increases Expression and Flux of Mg Transport Carriers Mediated by Activation of MEK/ERK/c-Fos Pathway in Renal Tubular Epithelial Cells.(2018) Nutrients. Sep 20 ;10(10)Read more>>
  • Wang JN et al. RIPK1 inhibitor Cpd-71 attenuates renal dysfunction in cisplatin-treated mice via attenuating necroptosis, inflammation and oxidative stress. Clin Sci (Lond). 2019 Jul 25;133(14):1609-1627. Read more>>
  • Wang JN et al. Smad3 promotes AKI sensitivity in diabetic mice via interaction with p53 and induction of NOX4-dependent ROS production. Redox Biol. 2020 Feb 26;32:101479.Read more>>
  • Ma Q et al. Non‐coenzyme role of vitamin B1 in RANKL‐induced osteoclastogenesis and ovariectomy induced osteoporosis. J Cell Biochem. 2020 Feb 26.Read more>>
  • Xue-Qi Liuet al. Rutaecarpine Derivative Cpd-6c Alleviates Acute Kidney Injury by Targeting PDE4B, a Key Enzyme Mediating inflammation in Cisplatin Nephropathy. Biochem Pharmacol. 2020 Oct;180:114132.Read more>>
  • Svetlana V Kostyuket al. Effects of Functionalized Fullerenes on ROS Homeostasis Determine Their Cytoprotective or Cytotoxic Properties. Nanomaterials (Basel). 2020 Jul 19;10(7):1405.Read more>>
  • Cheng Yu. et al. Exposure to maternal diabetes induces endothelial dysfunction and hypertension in adult male rat offspring. Microvasc Res. 2021 Jan;133:104076Read more>>
  • Jing Zhao. et al. Bruton's tyrosine kinase regulates macrophage?induced inflammation in the diabetic kidney via NLRP3 inflammasome activation. Int J Mol Med. 2021 Sep;48(3):1-12Read more>>
  • Jiang, Wenjuan. et al. Macrophage-derived, LRG1-enriched extracellular vesicles exacerbate aristolochic acid nephropathy in a TGFR1-dependent manner. 2021 Oct 22Read more>>
  • Peng Ding. et al. BMPER alleviates ischemic brain injury by protecting neurons and inhibiting neuroinflammation via Smad3-Akt-Nrf2 pathway. 2021 Dec 14Read more>>
  • Hu, Shuang. et al. Deletion of p38 attenuates ethanol consumption- and acetaminophen-induced liver injury in mice through promoting Dlg1. Acta Pharmacol Sin. 2021 Nov;:1-16Read more>>
  • Meiqiong Wu. et al. Suppression of NADPH oxidase 4 inhibits PM2.5-induced cardiac fibrosis through ROS-P38 MAPK pathway. SCI TOTAL ENVIRON. 2022 Apr;:155558Read more>>
  • Yan Li. et al. Inhibition of Nicotinamide adenine dinucleotide phosphate oxidase 4 attenuates cell apoptosis and oxidative stress in a rat model of polycystic ovary syndrome through the activation of Nrf-2/HO-1 signaling pathway. MOL CELL ENDOCRINOL. Mol Cell Endocrinol. 2022 Apr;:111645Read more>>
  • Wang Dan. et al. The Effect of Aerobic Exercise on the Oxidative Capacity of Skeletal Muscle Mitochondria in Mice with Impaired Glucose Tolerance. J DIABETES RES. 2022;2022:3780156Read more>>
  • Xu Zhou. et al. Magnesium isoglycyrrhizinate ameliorates lipopolysaccharide-induced liver injury by upregulating autophagy and inhibiting inflammation via IL-22 expression. BIOORG CHEM. 2022 Nov;128:106034Read more>>
  • Svetlana V. Kostyuk. et al. In Vitro Analysis of Biological Activity of Circulating Cell-Free DNA Isolated from Blood Plasma of Schizophrenic Patients and Healthy Controls—Part 2: Adaptive Response. GENES-BASEL. 2022 Dec;13(12):2283Read more>>
  • Pu Tian-Tian. et al. Evaluation of coenzyme Q10 (CoQ10) deficiency and therapy in mouse models of cardiomyopathy. J CARDIOVASC PHARM. 2023 Jan;:10.1097/FJC.0000000000001401Read more>>
  • Zheng-Hao Sun. et al. Interruption of TRPC6-NFATC1 signaling inhibits NADPH oxidase 4 and VSMCs phenotypic switch in intracranial aneurysm. BIOMED PHARMACOTHER. 2023 May;161:11448Read more>>
  • Guo-Jian Jiang. et al. Carteolol triggers senescence via activation of -arrestinCERKCNOX4CROS pathway in human corneal endothelial cells in vitro. CHEM-BIOL INTERACT. 2023 Apr;:110511Read more>>
  • Xin Guo. et al. Aspirin protects human trophoblast HTR-8/SVneo cells from H2O2-Induced oxidative stress via NADPH/ROS pathway. PLACENTA. 2023 Dec;144:55Read more>>
VALIDATION IMAGES