bs-1091R
[Primary Antibody]
NADPH oxidase 4 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: NADPH oxidase 4
Immunogen Range: 81-180/578
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 50490
Swiss Prot: Q9JHI8
Source: KLH conjugated synthetic peptide derived from mouse Nox-4
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
IP(1-2ug)
ICC(1:100-500)
Predicted Molecular Weight: 64
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Horse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Ma Q et al. Non‐coenzyme role of vitamin B1 in RANKL‐induced osteoclastogenesis and ovariectomy induced osteoporosis. J Cell Biochem. 2020 Feb 26.Read more>>
- Ulmasov et al. CLIC1 null mice demonstrate a role for CLIC1 in macrophage superoxide production and tissue injury. (2017) Physiol.Rep. 5 Read more>>
- Wang R et al. Leonurine alleviates LPS-induced myocarditis through suppressing the NF-кB signaling pathway. Toxicology. 2019 Apr 18;422:1-13.Read more>>
- Takashina Y et al. Sodium Citrate Increases Expression and Flux of Mg Transport Carriers Mediated by Activation of MEK/ERK/c-Fos Pathway in Renal Tubular Epithelial Cells.(2018) Nutrients. Sep 20 ;10(10)Read more>>
- Wang JN et al. RIPK1 inhibitor Cpd-71 attenuates renal dysfunction in cisplatin-treated mice via attenuating necroptosis, inflammation and oxidative stress. Clin Sci (Lond). 2019 Jul 25;133(14):1609-1627. Read more>>
- Wang JN et al. Smad3 promotes AKI sensitivity in diabetic mice via interaction with p53 and induction of NOX4-dependent ROS production. Redox Biol. 2020 Feb 26;32:101479.Read more>>
- Ma Q et al. Non‐coenzyme role of vitamin B1 in RANKL‐induced osteoclastogenesis and ovariectomy induced osteoporosis. J Cell Biochem. 2020 Feb 26.Read more>>
- Xue-Qi Liuet al. Rutaecarpine Derivative Cpd-6c Alleviates Acute Kidney Injury by Targeting PDE4B, a Key Enzyme Mediating inflammation in Cisplatin Nephropathy. Biochem Pharmacol. 2020 Oct;180:114132.Read more>>
- Svetlana V Kostyuket al. Effects of Functionalized Fullerenes on ROS Homeostasis Determine Their Cytoprotective or Cytotoxic Properties. Nanomaterials (Basel). 2020 Jul 19;10(7):1405.Read more>>
- Cheng Yu. et al. Exposure to maternal diabetes induces endothelial dysfunction and hypertension in adult male rat offspring. Microvasc Res. 2021 Jan;133:104076Read more>>
- Jing Zhao. et al. Bruton's tyrosine kinase regulates macrophage?induced inflammation in the diabetic kidney via NLRP3 inflammasome activation. Int J Mol Med. 2021 Sep;48(3):1-12Read more>>
- Jiang, Wenjuan. et al. Macrophage-derived, LRG1-enriched extracellular vesicles exacerbate aristolochic acid nephropathy in a TGFR1-dependent manner. 2021 Oct 22Read more>>
- Peng Ding. et al. BMPER alleviates ischemic brain injury by protecting neurons and inhibiting neuroinflammation via Smad3-Akt-Nrf2 pathway. 2021 Dec 14Read more>>
- Hu, Shuang. et al. Deletion of p38 attenuates ethanol consumption- and acetaminophen-induced liver injury in mice through promoting Dlg1. Acta Pharmacol Sin. 2021 Nov;:1-16Read more>>
- Meiqiong Wu. et al. Suppression of NADPH oxidase 4 inhibits PM2.5-induced cardiac fibrosis through ROS-P38 MAPK pathway. SCI TOTAL ENVIRON. 2022 Apr;:155558Read more>>
- Yan Li. et al. Inhibition of Nicotinamide adenine dinucleotide phosphate oxidase 4 attenuates cell apoptosis and oxidative stress in a rat model of polycystic ovary syndrome through the activation of Nrf-2/HO-1 signaling pathway. MOL CELL ENDOCRINOL. Mol Cell Endocrinol. 2022 Apr;:111645Read more>>
- Wang Dan. et al. The Effect of Aerobic Exercise on the Oxidative Capacity of Skeletal Muscle Mitochondria in Mice with Impaired Glucose Tolerance. J DIABETES RES. 2022;2022:3780156Read more>>
- Xu Zhou. et al. Magnesium isoglycyrrhizinate ameliorates lipopolysaccharide-induced liver injury by upregulating autophagy and inhibiting inflammation via IL-22 expression. BIOORG CHEM. 2022 Nov;128:106034Read more>>
- Svetlana V. Kostyuk. et al. In Vitro Analysis of Biological Activity of Circulating Cell-Free DNA Isolated from Blood Plasma of Schizophrenic Patients and Healthy Controls—Part 2: Adaptive Response. GENES-BASEL. 2022 Dec;13(12):2283Read more>>
- Pu Tian-Tian. et al. Evaluation of coenzyme Q10 (CoQ10) deficiency and therapy in mouse models of cardiomyopathy. J CARDIOVASC PHARM. 2023 Jan;:10.1097/FJC.0000000000001401Read more>>
- Zheng-Hao Sun. et al. Interruption of TRPC6-NFATC1 signaling inhibits NADPH oxidase 4 and VSMCs phenotypic switch in intracranial aneurysm. BIOMED PHARMACOTHER. 2023 May;161:11448Read more>>
- Guo-Jian Jiang. et al. Carteolol triggers senescence via activation of -arrestinCERKCNOX4CROS pathway in human corneal endothelial cells in vitro. CHEM-BIOL INTERACT. 2023 Apr;:110511Read more>>
- Xin Guo. et al. Aspirin protects human trophoblast HTR-8/SVneo cells from H2O2-Induced oxidative stress via NADPH/ROS pathway. PLACENTA. 2023 Dec;144:55Read more>>
VALIDATION IMAGES
Mouse muscle lysates probed with Rabbit Anti-NADPH oxidase 4 Polyclonal Antibody, Unconjugated (bs-1091R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Nox4/NADH Polyclonal Antibody, Unconjugated (bs-1091R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Human HUVEC cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human U251 cell lysates probed with NADPH oxidase 4 Polyclonal Antibody, Unconjugated (bs-1091R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
293T cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NADPH oxidase 4 Antibody(bs-1091R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Mouse Bronchus tissue lysates; Lane 4: Mouse Heart tissue lysates ; Lane 5: Rat Kidney tissue lysates; Lane 6: Rat Lung tissue lysates; Lane 7: Rat Bronchus tissue lysates probed with NADPH oxidase 4 Polyclonal Antibody, Unconjugated (bs-1091R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NADPH oxidase 4 Polyclonal Antibody, Unconjugated (bs-1091R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NADPH oxidase 4 Polyclonal Antibody, Unconjugated (bs-1091R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NADPH oxidase 4 Polyclonal Antibody, Unconjugated (bs-1091R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NADPH oxidase 4 Polyclonal Antibody, Unconjugated (bs-1091R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NADPH oxidase 4 Polyclonal Antibody, Unconjugated (bs-1091R) at 1:200 overnight at 4°C, DAB staining.