Host: Rabbit
Target Protein: Caspase-1 p20
Immunogen Range: 101-200/404
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 834
Swiss Prot: P29466
Source: KLH conjugated synthetic peptide derived from human Caspase-1 p20
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 20/46
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
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- Xiaohui Wang. et al. Short-wavelength blue light contributes to the pyroptosis of human lens epithelial cells (hLECs). Exp Eye Res. 2021 Nov;212:108786Read more>>
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- Xiang Wu. et al. NLRP3 inflammasome inhibitor MCC950 reduces cerebral ischemia/reperfusion induced neuronal ferroptosis. NEUROSCI LETT. 2023 Jan;795:137032Read more>>
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- Shan Cao. et al. Study on the therapeutic mechanism of HJ granules in a rat model of urinary tract infection caused by Escherichia coli. J ETHNOPHARMACOL. 2024 Mar;:118056Read more>>
- Yongmei Peng. et al. Mechanism of Vaginal Epithelial Cell Pyroptosis Induced by the NLRP3 Inflammasome in Vulvovaginal Candidiasis. AM J REPROD IMMUNOL. 2024 Jul;92(1):e13893Read more>>
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37Ā°C for 20min; Antibody incubation with Caspase-1 p20 Polyclonal Antibody, Unconjugated (bs-10743R) at 1:200 overnight at 4Ā°C, followed by conjugation to the secondary antibody and DAB staining
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Caspase-1 p20 Polyclonal Antibody(bs-10743R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).